Effect of NusA protein on expression of the nusA,infB operon in E. coli.
Protein and operon fusions between lacZ and various genes of the nusA,infB operon have been constructed on lambda bacteriophages and used to show that the operon is negatively regulated by the level of NusA protein. Overproducing NusA (but not IF2) from a multicopy plasmid reduces the level of beta-galactosidase from the fusions indicating repression of the operon. Introducing the lambda carrying the fusions into nusA mutant strains produces a higher level of beta-galactosidase-indicative of derepression of the operon. In particular, a larger form of the NusA protein which does not affect bacterial growth per se causes a derepression of the operon. As both protein and operon fusions respond equivalently, we conclude that the nusA protein is acting at the transcriptional level to regulate expression of the nusA, infB operon.
26 references, click to expand
-
Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli.J Bacteriol. 1977 Jan;129(1):378-87 PMID: 318645
-
The construction in vitro of transducing derivatives of phage lambda.Mol Gen Genet. 1976 Jul 23;146(2):199-207 PMID: 785220
-
L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination.Proc Natl Acad Sci U S A. 1980 Apr;77(4):1991-4 PMID: 6154941
-
Selection for loss of tetracycline resistance by Escherichia coli.J Bacteriol. 1981 Feb;145(2):1110-1 PMID: 7007341
-
In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
-
Termination of transcription by nusA gene protein of Escherichia coli.Nature. 1981 Jul 16;292(5820):215-20 PMID: 6265785
-
The nusA gene protein of Escherichia coli. Its identification and a demonstration that it interacts with the gene N transcription anti-termination protein of bacteriophage lambda.J Mol Biol. 1981 Mar 25;147(1):11-23 PMID: 6455533
-
Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters.Cell. 1981 Sep;25(3):773-82 PMID: 6793240
-
Cloning of E. coli pnp gene from an episome.Mol Gen Genet. 1981;183(2):298-305 PMID: 6276682
-
Regulation of tryptophanyl-tRNA synthetase formation.J Bacteriol. 1982 Aug;151(2):918-23 PMID: 7047500
-
Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli.Proc Natl Acad Sci U S A. 1982 Aug;79(16):5033-7 PMID: 6214789
-
In vitro construction of the tufB-lacZ fusion: analysis of the regulatory mechanism of tufB promoter.Mol Gen Genet. 1982;187(3):355-63 PMID: 6294467
-
Initiation factor and ribosome levels are coordinately controlled in Escherichia coli growing at different rates.J Biol Chem. 1983 Feb 10;258(3):1954-9 PMID: 6337147
-
Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.Gene. 1982 Dec;20(2):305-16 PMID: 6299895
-
Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA).J Mol Biol. 1983 Jun 25;167(2):227-43 PMID: 6306257
-
Isolation of conditionally lethal amber mutations affecting synthesis of the nusA protein of Escherichia coli.Mol Gen Genet. 1983;190(2):196-203 PMID: 6308387
-
Structural and transcriptional evidence for related thrS and infC expression.Proc Natl Acad Sci U S A. 1983 Oct;80(20):6152-6 PMID: 6353409
-
Escherichia coli phenylalanyl-tRNA synthetase operon is controlled by attenuation in vivo.J Mol Biol. 1983 Dec 15;171(3):263-79 PMID: 6317866
-
tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.Proc Natl Acad Sci U S A. 1984 Jan;81(2):409-13 PMID: 6364142
-
Amplification and isolation of Escherichia coli nusA protein and studies of its effects on in vitro RNA chain elongation.Biochemistry. 1984 Jan 17;23(2):197-203 PMID: 6199039
-
The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli.Nucleic Acids Res. 1984 Apr 11;12(7):3333-42 PMID: 6326058
-
Expression of the rpsO and pnp genes: structural analysis of a DNA fragment carrying their control regions.Nucleic Acids Res. 1984 Aug 10;12(15):6091-102 PMID: 6382163
-
Two translational initiation sites in the infB gene are used to express initiation factor IF2 alpha and IF2 beta in Escherichia coli.EMBO J. 1985 Jan;4(1):223-9 PMID: 3894004
-
Pausing and attenuation of in vitro transcription in the rrnB operon of E. coli.Cell. 1981 Dec;27(3 Pt 2):523-31 PMID: 6086107
-
Safer derivatives of bacteriophage lambdagt-lambdaC for use in cloning of recombinant DNA molecules.Nature. 1976 Feb 19;259(5544):596-8 PMID: 765843
-
Description and properties of bacteriophage lambda vectors useful for the cloning of EcoRI DNA fragments.Biochimie. 1978;60(2):183-7 PMID: 352409
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