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PMID: 1421569 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inhibition of c-Jun DNA binding by mitogen-activated protein kinase.

Molecular biology of the cell ·Vol. 3 ·No. 10 ·1992-10-00 ·Pages 1117-30

Chou SY, Baichwal V, Ferrell JE

Abstract

Here we demonstrate that partially purified Xenopus p42 mitogen-activated protein (MAP) kinase phosphorylates bacterially expressed human c-Jun at a single site, serine 243. Several lines of evidence argue that this phosphorylation is due to p42 MAP kinase itself rather than some contaminating species. Phosphorylation of serine 243 markedly decreases the binding of c-Jun to oligonucleotides containing the 12-O-tetradecanoylphorbol-13-acetate response element. These findings suggest that MAP kinase may play a role in the down-regulation of c-Jun or in the cycle of transcriptional initiation and elongation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites DNA/genetics,metabolism Female Humans Mitogen-Activated Protein Kinase 1 Molecular Sequence Data Oocytes/metabolism Phosphorylation Protein Serine-Threonine Kinases/isolation & purification,metabolism Protein-Tyrosine Kinases/isolation & purification,metabolism Proto-Oncogene Proteins c-jun/genetics,metabolism Xenopus laevis
Chemicals
Proto-Oncogene Proteins c-jun DNA Protein-Tyrosine Kinases Protein Serine-Threonine Kinases Mitogen-Activated Protein Kinase 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chou S Y
Department of Zoology, University of Wisconsin, Madison 53706.
Baichwal V
Ferrell J E
References (63)
63 references, click to expand
  1. A purified S6 kinase kinase from Xenopus eggs activates S6 kinase II and autophosphorylates on serine, threonine, and tyrosine residues.
    J Biol Chem. 1992 Mar 5;267(7):4408-15 PMID: 1311309
  2. c-Kit-kinase induces a cascade of protein tyrosine phosphorylation in normal human melanocytes in response to mast cell growth factor and stimulates mitogen-activated protein kinase but is down-regulated in melanomas.
    Mol Biol Cell. 1992 Feb;3(2):197-209 PMID: 1372524
  3. Nerve growth factor stimulates the tyrosine phosphorylation of MAP2 kinase in PC12 cells.
    Neuron. 1991 Jun;6(6):915-22 PMID: 1647176
  4. Sequence of pp42/MAP kinase, a serine/threonine kinase regulated by tyrosine phosphorylation.
    Nucleic Acids Res. 1991 Jul 11;19(13):3743 PMID: 1649458
  5. Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.
    Cell Regul. 1991 May;2(5):357-71 PMID: 1654126
  6. A 42-kD tyrosine kinase substrate linked to chromaffin cell secretion exhibits an associated MAP kinase activity and is highly related to a 42-kD mitogen-stimulated protein in fibroblasts.
    J Cell Biol. 1990 Mar;110(3):731-42 PMID: 1689732
  7. In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
    Nature. 1991 Jan 17;349(6306):251-4 PMID: 1702878
  8. Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
    Mol Cell Biol. 1991 May;11(5):2517-28 PMID: 1708093
  9. Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
    EMBO J. 1991 Sep;10(9):2661-8 PMID: 1714387
  10. Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.
    Biochemistry. 1991 Jan 8;30(1):278-86 PMID: 1846291
  11. Epidermal growth factor (EGF) receptor T669 peptide kinase from 3T3-L1 cells is an EGF-stimulated "MAP" kinase.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2520-4 PMID: 1848706
  12. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain.
    Nature. 1991 May 9;351(6322):122-7 PMID: 1903181
  13. Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases.
    J Biol Chem. 1991 Nov 25;266(33):22159-63 PMID: 1939237
  14. Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
    Mol Cell Biol. 1991 Apr;11(4):1965-71 PMID: 2005892
  15. Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.
    J Biol Chem. 1990 Jul 15;265(20):11487-94 PMID: 2142153
  16. Stimulation of MAP-2 kinase activity in T lymphocytes by anti-CD3 or anti-Ti monoclonal antibody is partially dependent on protein kinase C.
    J Immunol. 1990 Apr 1;144(7):2683-9 PMID: 2156931
  17. pp54 microtubule-associated protein 2 kinase. A novel serine/threonine protein kinase regulated by phosphorylation and stimulated by poly-L-lysine.
    J Biol Chem. 1990 Oct 5;265(28):17355-63 PMID: 2170374
  18. Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
    Eur J Biochem. 1990 Nov 13;193(3):661-9 PMID: 2174361
  19. Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
    J Biol Chem. 1990 Jan 5;265(1):52-7 PMID: 2294120
  20. Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
    J Biol Chem. 1988 Feb 5;263(4):2009-19 PMID: 2448302
  21. Biochemical analysis of transcriptional activation by Jun: differential activity of c- and v-Jun.
    Cell. 1989 Nov 17;59(4):709-17 PMID: 2510934
  22. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae.
    Cell. 1989 Sep 22;58(6):1107-19 PMID: 2673544
  23. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  24. Nonenzymatic phosphorylation of tyrosine and serine by ATP is catalyzed by manganese but not magnesium.
    J Biol Chem. 1988 Oct 25;263(30):15590-3 PMID: 3139672
  25. Diverse mitogenic agents induce rapid phosphorylation of a common set of cellular proteins at tyrosine in quiescent mammalian cells.
    J Biol Chem. 1985 Feb 10;260(3):1771-9 PMID: 3871438
  26. Phorbol ester and diacylglycerol induce protein phosphorylation at tyrosine.
    Nature. 1983 Dec 1-7;306(5942):487-90 PMID: 6196643
  27. Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
    Science. 1992 Jan 10;255(5041):212-5 PMID: 1313186
  28. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.
    Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2365-9 PMID: 1347949
  29. Nuclear localization and regulation of erk- and rsk-encoded protein kinases.
    Mol Cell Biol. 1992 Mar;12(3):915-27 PMID: 1545823
  30. Related proteins are phosphorylated at tyrosine in response to mitogenic stimuli and at meiosis.
    Mol Cell Biol. 1989 Jul;9(7):3143-7 PMID: 2779558
  31. Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
    Proc Natl Acad Sci U S A. 1987 Mar;84(6):1502-6 PMID: 2951732
  32. Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.
    J Biol Chem. 1988 Apr 15;263(11):5396-401 PMID: 3258598
  33. Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast.
    Cell. 1986 Sep 12;46(6):885-94 PMID: 3530496
  34. Similar effects of platelet-derived growth factor and epidermal growth factor on the phosphorylation of tyrosine in cellular proteins.
    Cell. 1982 Nov;31(1):263-73 PMID: 6186382
  35. Diverse mitogenic agents induce the phosphorylation of two related 42,000-dalton proteins on tyrosine in quiescent chick cells.
    Mol Cell Biol. 1984 Jan;4(1):30-7 PMID: 6199661
  36. A tumor promoter stimulates phosphorylation on tyrosine.
    Biochem Biophys Res Commun. 1983 Sep 15;115(2):536-43 PMID: 6414472
  37. Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase.
    J Biol Chem. 1990 Nov 15;265(32):19728-35 PMID: 1700979
  38. Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.
    J Biol Chem. 1991 Mar 5;266(7):4220-7 PMID: 1705548
  39. Activation of mitogen-activated protein kinase in BC3H1 myocytes by fluoroaluminate.
    J Biol Chem. 1991 Jun 5;266(16):10131-5 PMID: 1709925
  40. Preparation and use of anti-phosphotyrosine antibodies to study structure and function of insulin receptor.
    Methods Enzymol. 1991;201:65-79 PMID: 1719349
  41. An array of insulin-activated, proline-directed serine/threonine protein kinases phosphorylate the p70 S6 kinase.
    J Biol Chem. 1992 Feb 15;267(5):3325-35 PMID: 1737788
  42. Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity.
    Cell. 1991 Feb 8;64(3):573-84 PMID: 1846781
  43. Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).
    EMBO J. 1991 Apr;10(4):885-92 PMID: 1849075
  44. Nerve growth factor stimulates protein tyrosine phosphorylation in PC-12 pheochromocytoma cells.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2653-7 PMID: 1849270
  45. Phosphorylation of c-jun mediated by MAP kinases.
    Nature. 1991 Oct 17;353(6345):670-4 PMID: 1922387
  46. Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates.
    Methods Enzymol. 1991;201:110-49 PMID: 1943760
  47. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.
    Cell. 1991 May 17;65(4):663-75 PMID: 2032290
  48. Control of c-Jun activity by interaction of a cell-specific inhibitor with regulatory domain delta: differences between v- and c-Jun.
    Cell. 1990 Nov 16;63(4):815-25 PMID: 2121368
  49. Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
    Nature. 1990 Feb 15;343(6259):651-3 PMID: 2154696
  50. An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control.
    Science. 1990 Jul 6;249(4964):64-7 PMID: 2164259
  51. Okadaic acid mimics the action of insulin in stimulating protein kinase activity in isolated adipocytes. The role of protein phosphatase 2a in attenuation of the signal.
    J Biol Chem. 1990 Sep 25;265(27):16571-80 PMID: 2168895
  52. Evidence for communication between nerve growth factor and protein tyrosine phosphorylation.
    FEBS Lett. 1990 Oct 1;271(1-2):119-22 PMID: 2171996
  53. Phosphorylation of Xenopus cyclins B1 and B2 is not required for cell cycle transitions.
    Mol Cell Biol. 1991 Aug;11(8):3860-7 PMID: 1649383
  54. Isolation and characterization of two growth factor-stimulated protein kinases that phosphorylate the epidermal growth factor receptor at threonine 669.
    J Biol Chem. 1991 Aug 15;266(23):15266-76 PMID: 1651322
  55. Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase.
    J Biol Chem. 1991 Aug 15;266(23):15277-85 PMID: 1651323
  56. Biochemical characterization of a family of serine/threonine protein kinases regulated by tyrosine and serine/threonine phosphorylations.
    J Biol Chem. 1991 Oct 25;266(30):20270-5 PMID: 1657919
  57. Identification of a 42-kilodalton phosphotyrosyl protein as a serine(threonine) protein kinase by renaturation.
    Mol Cell Biol. 1990 Jun;10(6):3020-6 PMID: 1692963
  58. Okadaic acid activates microtubule-associated protein kinase in quiescent fibroblastic cells.
    Eur J Biochem. 1990 Nov 13;193(3):671-4 PMID: 2174362
  59. Nerve growth factor and fibroblast growth factor selectively activate a protein kinase that phosphorylates high molecular weight microtubule-associated proteins. Detection, partial purification, and characterization in PC12 cells.
    J Biol Chem. 1990 Sep 15;265(26):15471-80 PMID: 2394735
  60. FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
    Cell. 1990 Feb 23;60(4):649-64 PMID: 2406028
  61. Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
    Science. 1989 Mar 31;243(4899):1689-94 PMID: 2494701
  62. Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6940-3 PMID: 2550926
  63. Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2.
    Nature. 1989 Jun 29;339(6227):679-84 PMID: 2662013
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.3.10.1117
Published
1992-10-00
Pages
1117-30
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275676
Subset
IM
Grants
NIGMS NIH HHS · GM-46383 · United States
External Links
PubMed source DOI: 10.1091/mbc.3.10.1117 PMC Full Text View journal details
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