Preparation and use of anti-phosphotyrosine antibodies to study structure and function of insulin receptor.

Animals Antibodies/isolation & purification CHO Cells Cell Line Chromatography, Affinity/methods Cricetinae Electrophoresis, Polyacrylamide Gel/methods Humans Immunoblotting/methods Indicators and Reagents Insulin/pharmacology Liver Neoplasms, Experimental Macromolecular Substances Molecular Weight Neoplasm Proteins/metabolism Phosphates/metabolism Phosphatidylinositol 3-Kinases Phosphoproteins/isolation & purification,metabolism Phosphorus Radioisotopes Phosphotransferases/metabolism Phosphotyrosine Protein-Tyrosine Kinases/isolation & purification,metabolism Rabbits/immunology Receptor, Insulin/isolation & purification,metabolism Transfection Tyrosine/analogs & derivatives,analysis,immunology

Antibodies Indicators and Reagents Insulin Macromolecular Substances Neoplasm Proteins Phosphates Phosphoproteins Phosphorus Radioisotopes Phosphotyrosine Tyrosine Phosphotransferases Phosphatidylinositol 3-Kinases Protein-Tyrosine Kinases Receptor, Insulin