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PMID: 1406685 Published · ppublish English Journal Article

A Drosophila rotund transcript expressed during spermatogenesis and imaginal disc morphogenesis encodes a protein which is similar to human Rac GTPase-activating (racGAP) proteins.

Molecular and cellular biology ·Vol. 12 ·No. 11 ·1992-11-00 ·Pages 5111-22

Agnel M, Röder L, Vola C, Griffin-Shea R

Abstract

The rotund (rn) locus of Drosophila melanogaster at cytogenetic position 84D3,4 has been isolated and cloned on the basis of the mutant phenotype: an absence of structures in the subdistal regions of the appendages. The shortened appendages are the consequence of a localized cell death in the imaginal discs, precursors of the adult appendages. Physical characterization of the rn locus has demonstrated that it is relatively large, occupying a minimum of 50 kb. There are two major transcripts of 1.7 kb (m1.7) and 5.3 kb (m5.3). We present here the sequence analysis of m1.7 and its putative product, rnprot1.7, and show that rnprot1.7 is similar to the product of the human n-chimaerin gene, which is expressed in brain and testes. Recently, the GAP activity of n-chimaerin was demonstrated and shown to be specific for the Rac subfamily of the Ras oncoproteins. The Rac proteins have been implicated in the regulation of secretory processes. In addition to being expressed in the imaginal discs, the m1.7 racGAP transcript was detected in developmentally specific germ line cells of the testes, the primary spermatocytes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Drosophila melanogaster/genetics Exons Extremities/embryology GTP-Binding Proteins/genetics,metabolism GTPase-Activating Proteins Humans Male Molecular Sequence Data Morphogenesis/genetics Proteins/genetics,metabolism Sequence Homology, Amino Acid Spermatogenesis/genetics Transcription, Genetic rac GTP-Binding Proteins ras GTPase-Activating Proteins
Chemicals
GTPase-Activating Proteins Proteins ras GTPase-Activating Proteins DNA GTP-Binding Proteins rac GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Agnel M
Laboratoire de Génétique et Biologie Cellulaires, Centre National de la Recherche Scientifique, Marseille, France.
Röder L
Vola C
Griffin-Shea R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.11.5111-5122.1992
Published
1992-11-00
Pages
5111-22
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360445
Subset
IM
Databases
GENBANK
M99702
External Links
PubMed source DOI: 10.1128/mcb.12.11.5111-5122.1992 PMC Full Text View journal details
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