Home LiteratureArticle Details
PMID: 1400197 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rhodobacter sphaeroides rdxA, a homolog of Rhizobium meliloti fixG, encodes a membrane protein which may bind cytoplasmic [4Fe-4S] clusters.

Journal of bacteriology ·Vol. 174 ·No. 20 ·1992-10-00 ·Pages 6444-54

Neidle EL, Kaplan S

Abstract

In the photosynthetic bacterium Rhodobacter sphaeroides, a chromosomal gene, rdxA, which encodes a 52-kDa protein, was found to be homologous to fixG, the first gene of a Rhizobium meliloti nitrogen fixation operon on the pSym plasmid (D. Kahn, M. David, O. Domergue, M.-L. Daveran, J. Ghai, P. R. Hirsch, and J. Batut, J. Bacteriol. 171:929-939, 1989). The deduced amino acid sequences of RdxA and FixG are 53% identical and 73% similar; sequence analyses suggested that each has five transmembrane helices and a central region resembling bacterial-type ferredoxins. Translational fusion proteins with an alkaline phosphatase reporter group were expressed in both R. sphaeroides and Escherichia coli and were used to assess the membrane topology of RdxA. Its ferredoxinlike sequence, which may bind two [4Fe-4S] centers, was found to be cytoplasmically located. Genetic disruptions showed that rdxA is not essential for nitrogen fixation in R. sphaeroides. Immediately downstream of rdxA, an open reading frame (ORFT2) that encoded a 48-kDa protein was found. This DNA sequence was not homologous to any region of the R. meliloti fixG operon. The N-terminal sequence of the ORFT2 gene product resembled amino acid sequences found in members of the GntR family of regulatory proteins (D. J. Haydon and J. R. Guest, FEMS Microbiol. Lett. 79:291-296, 1991). The rdxA gene was localized to the smaller of two R. sphaeroides chromosomes, upstream of and divergently transcribed from hemT, which encodes one of two 5-aminolevulinate synthase isozymes. The rdxA and hemT genes may share a transcriptional regulatory region. Southern hybridization analysis demonstrated the presence of an rdxA homolog on the R. sphaeroides large chromosome. The functions of this homolog, like those of rdxA, remain to be determined, but roles in oxidation-reduction processes are likely.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Base Sequence Blotting, Southern Genes, Bacterial/genetics Iron-Sulfur Proteins/chemistry,genetics,metabolism Membrane Proteins/chemistry,genetics,metabolism Molecular Sequence Data Mutagenesis/genetics Plasmids/genetics Recombinant Fusion Proteins/chemistry,genetics,metabolism Rhodobacter sphaeroides/genetics Sequence Homology, Nucleic Acid Sinorhizobium meliloti/genetics
Chemicals
Bacterial Proteins Iron-Sulfur Proteins Membrane Proteins Recombinant Fusion Proteins RdxA protein, Rhodobacter sphaeroides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Neidle E L
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston 77225.
Kaplan S
References (61)
61 references, click to expand
  1. A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti.
    Nature. 1991 Mar 14;350(6314):170-2 PMID: 1848683
  2. Sequences of six genes and several open reading frames in the kinetoplast maxicircle DNA of Leishmania tarentolae.
    J Biol Chem. 1984 Dec 25;259(24):15136-47 PMID: 6096360
  3. Intrinsically bent DNA.
    J Biol Chem. 1990 May 5;265(13):7093-6 PMID: 2185240
  4. Cloning and expression of three rabbit kidney cDNAs encoding lauric acid omega-hydroxylases.
    Biochemistry. 1990 Jan 30;29(4):873-9 PMID: 2340280
  5. Alkaline phosphatase fusions: sensors of subcellular location.
    J Bacteriol. 1990 Feb;172(2):515-8 PMID: 2404939
  6. Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation.
    J Bacteriol. 1989 Feb;171(2):929-39 PMID: 2536685
  7. Construction, expression, and localization of a CycA::PhoA fusion protein in Rhodobacter sphaeroides and Escherichia coli.
    J Bacteriol. 1989 Nov;171(11):5830-9 PMID: 2553661
  8. Characterization of two genes encoding human steroid 11 beta-hydroxylase (P-450(11) beta).
    J Biol Chem. 1989 Dec 15;264(35):20961-7 PMID: 2592361
  9. Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli.
    Nucleic Acids Res. 1988 Aug 25;16(16):7995-8009 PMID: 2843809
  10. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.
    Gene. 1988 Oct 15;70(1):191-7 PMID: 2853689
  11. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  12. Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex.
    J Bacteriol. 1987 Feb;169(2):864-73 PMID: 3027051
  13. Drosophila melanogaster mitochondrial DNA: gene organization and evolutionary considerations.
    Genetics. 1988 Apr;118(4):649-63 PMID: 3130291
  14. Nucleotide sequence and comparative analysis of the frd operon encoding the fumarate reductase of Proteus vulgaris. Extensive sequence divergence of the membrane anchors and absence of an frd-linked ampC cephalosporinase gene.
    Eur J Biochem. 1987 Sep 15;167(3):481-8 PMID: 3308458
  15. The detection and classification of membrane-spanning proteins.
    Biochim Biophys Acta. 1985 May 28;815(3):468-76 PMID: 3838905
  16. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences.
    Gene. 1984 Oct;30(1-3):157-66 PMID: 6096212
  17. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  18. The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide sequence encoding the pyruvate dehydrogenase component.
    Eur J Biochem. 1983 Jun 1;133(1):155-62 PMID: 6343085
  19. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  20. Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800).
    J Biol Chem. 1982 Dec 10;257(23):14192-7 PMID: 6754724
  21. Purification, some properties and amino acid sequence of Thermus thermophilus HB8 ferredoxin.
    Biochim Biophys Acta. 1981 Apr 28;668(2):277-89 PMID: 7225412
  22. THE CULTURE, GENERAL PHYSIOLOGY, MORPHOLOGY, AND CLASSIFICATION OF THE NON-SULFUR PURPLE AND BROWN BACTERIA.
    Bacteriol Rev. 1944 Mar;8(1):1-118 PMID: 16350090
  23. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Fate of "old" and "new" membrane.
    J Biol Chem. 1978 Jan 25;253(2):451-7 PMID: 304056
  24. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  25. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.
    J Mol Biol. 1975 Aug 5;96(2):307-16 PMID: 1100846
  26. Localization and structural analysis of the ribosomal RNA operons of Rhodobacter sphaeroides.
    Nucleic Acids Res. 1990 Dec 25;18(24):7267-77 PMID: 1701878
  27. cis-acting regulatory elements involved in oxygen and light control of puc operon transcription in Rhodobacter sphaeroides.
    J Bacteriol. 1992 Feb;174(4):1146-57 PMID: 1735709
  28. Nodules elicited by Rhizobium meliloti heme mutants are arrested at an early stage of development.
    Mol Gen Genet. 1991 Dec;230(3):423-32 PMID: 1766439
  29. Evolution of the cytochrome b gene of mammals.
    J Mol Evol. 1991 Feb;32(2):128-44 PMID: 1901092
  30. An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.
    Mol Gen Genet. 1990 Aug;223(1):138-47 PMID: 2175385
  31. Recent developments in the field of iron-sulfur proteins.
    FASEB J. 1990 May;4(8):2483-91 PMID: 2185975
  32. Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components.
    Mol Microbiol. 1990 Feb;4(2):231-43 PMID: 2187144
  33. Molecular cloning, primary structure, and expression of the human platelet/erythroleukemia cell 12-lipoxygenase.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5638-42 PMID: 2377602
  34. Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly, Luciola cruciata.
    Gene. 1989 Apr 30;77(2):265-70 PMID: 2473944
  35. The complete nucleotide sequence of the Rattus norvegicus mitochondrial genome: cryptic signals revealed by comparative analysis between vertebrates.
    J Mol Evol. 1989 Jun;28(6):497-516 PMID: 2504926
  36. Overexpression and site-directed mutagenesis of the succinyl-CoA synthetase of Escherichia coli and nucleotide sequence of a gene (g30) that is adjacent to the suc operon.
    Biochem J. 1989 Jun 15;260(3):737-47 PMID: 2548486
  37. Physical and genetic mapping of the Rhodobacter sphaeroides 2.4.1 genome: genome size, fragment identification, and gene localization.
    J Bacteriol. 1989 Nov;171(11):5840-9 PMID: 2553662
  38. Cloning of rabbit uricase cDNA reveals a conserved carboxy-terminal tripeptide in three species.
    Biochim Biophys Acta. 1989 Jun 1;1008(1):116-8 PMID: 2719958
  39. Physical and genetic mapping of the Rhodobacter sphaeroides 2.4.1 genome: presence of two unique circular chromosomes.
    J Bacteriol. 1989 Nov;171(11):5850-9 PMID: 2808300
  40. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  41. Primary structure of the two (4 Fe-4 S) clusters ferredoxin from Desulfovibrio desulfuricans (strain Norway 4).
    Biochimie. 1983 Jan;65(1):43-7 PMID: 6403056
  42. Structure of the extracellular ferredoxin from Rhodospirillum rubrum: close similarity to clostridial ferredoxins.
    J Biochem. 1983 May;93(5):1385-90 PMID: 6411697
  43. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  44. Complete amino acid sequence of the 4Fe-4S, thermostable ferredoxin from Clostridium thermoaceticum.
    Biochemistry. 1982 Jul 6;21(14):3294-8 PMID: 7115670
  45. Recognition of protein coding regions in DNA sequences.
    Nucleic Acids Res. 1982 Sep 11;10(17):5303-18 PMID: 7145702
  46. Sequence and gene organization of mouse mitochondrial DNA.
    Cell. 1981 Oct;26(2 Pt 2):167-80 PMID: 7332926
  47. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  48. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  49. Identification of intrinsic high-level resistance to rare-earth oxides and oxyanions in members of the class Proteobacteria: characterization of tellurite, selenite, and rhodium sesquioxide reduction in Rhodobacter sphaeroides.
    J Bacteriol. 1992 Mar;174(5):1505-14 PMID: 1537795
  50. Chromosome transfer in Rhodobacter sphaeroides: Hfr formation and genetic evidence for two unique circular chromosomes.
    J Bacteriol. 1992 Feb;174(4):1135-45 PMID: 1735708
  51. Cloning and characterization of a cDNA encoding the chloroplastic copper/zinc-superoxide dismutase from pea.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7661-5 PMID: 2845417
  52. A conformational preference parameter to predict helices in integral membrane proteins.
    Biochim Biophys Acta. 1986 Jan 30;869(2):197-214 PMID: 2935194
  53. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  54. Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
    J Biol Chem. 1986 Oct 15;261(29):13744-53 PMID: 3020045
  55. Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae.
    J Mol Biol. 1988 Oct 5;203(3):715-38 PMID: 3062178
  56. Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties.
    J Bacteriol. 1988 Oct;170(10):4634-51 PMID: 3170481
  57. Cloning and characterization of the 5-aminolevulinate synthase gene(s) from Rhodobacter sphaeroides.
    Gene. 1988 Oct 15;70(1):139-51 PMID: 3266489
  58. Systematic method for the detection of potential lambda Cro-like DNA-binding regions in proteins.
    J Mol Biol. 1987 Apr 5;194(3):557-64 PMID: 3625774
  59. A method to locate protein coding sequences in DNA of prokaryotic systems.
    Nucleic Acids Res. 1985 Jan 11;13(1):185-94 PMID: 3839071
  60. Amino acid sequence of a ferredoxin from thermoacidophilic archaebacterium, Sulfolobus acidocaldarius. Presence of an N6-monomethyllysine and phyletic consideration of archaebacteria.
    J Biochem. 1985 Mar;97(3):745-53 PMID: 3926756
  61. A new family of bacterial regulatory proteins.
    FEMS Microbiol Lett. 1991 Apr 15;63(2-3):291-5 PMID: 2060763
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.20.6444-6454.1992
Published
1992-10-00
Pages
6444-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207600
Subset
IM
Grants
NIGMS NIH HHS · GM13138 · United States
NIGMS NIH HHS · GM15590 · United States
Databases
GENBANK
M94060, M94061, M94062, M94629, M94692, M94725, X64378, X64379, X64380, X64381
External Links
PubMed source DOI: 10.1128/jb.174.20.6444-6454.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2