Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties.
The complete nucleotide sequence of the multicopy Streptomyces plasmid pIJ101 has been determined and correlated with previously published genetic data. The circular DNA molecule is 8,830 nucleotides in length and has a G+C composition of 72.98%. The use of a computer program, FRAME, enabled identification in the sequence of seven open reading frames, four of which, tra (621 amino acids [aa]), spdA (146 aa), spdB (274 aa), and kilB (177 aa), appear to be genes involved in plasmid transfer. At least two of the above genes are predicted to be transcribed by known promoters that are regulated in trans by the products of the korA (241 aa) and korB (80 aa) loci on the plasmid. The segment of the plasmid capable of autonomous replication contains one large open reading frame (rep; 450 aa) and a noncoding region presumed to be the origin of replication. Four other small (less than 90 aa) open reading frames are also present on the plasmid, although no function can be attributed to them. The sequence of the pIJ101 replication segment present in several widely used cloning vectors (e.g., pIJ350 and pIJ702) has also been determined, so that the complete nucleotide sequences of these vectors are now known.
28 references, click to expand
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
-
Prediction of the secondary structure of proteins from their amino acid sequence.Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 PMID: 364941
-
pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.Mol Gen Genet. 1982;185(2):223-8 PMID: 6283316
-
Cloning of antibiotic resistance and nutritional genes in streptomycetes.J Bacteriol. 1982 Aug;151(2):668-77 PMID: 6284706
-
The helical hydrophobic moment: a measure of the amphiphilicity of a helix.Nature. 1982 Sep 23;299(5881):371-4 PMID: 7110359
-
Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans.Mol Gen Genet. 1982;187(2):265-77 PMID: 6294463
-
New M13 vectors for cloning.Methods Enzymol. 1983;101:20-78 PMID: 6310323
-
Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.J Gen Microbiol. 1983 Jul;129(7):2257-69 PMID: 6631413
-
Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.J Gen Microbiol. 1983 Sep;129(9):2703-14 PMID: 6313861
-
Regulation of plasmid replication.Microbiol Rev. 1984 Mar;48(1):1-23 PMID: 6201704
-
Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.EMBO J. 1982;1(8):945-51 PMID: 6329717
-
Protein-DNA recognition.Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
-
The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences.Gene. 1984 Oct;30(1-3):157-66 PMID: 6096212
-
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene. 1985;33(1):103-19 PMID: 2985470
-
Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces.Mol Gen Genet. 1985;199(1):26-36 PMID: 2987648
-
Conjugative sex plasmids of Streptomyces.Basic Life Sci. 1985;30:615-34 PMID: 2990430
-
RNA polymerase-DNA interactions in Streptomyces. In vitro studies of a S. lividans plasmid promoter with S. coelicolor RNA polymerase.J Mol Biol. 1985 Sep 5;185(1):177-88 PMID: 4046037
-
The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product.Gene. 1985;37(1-3):101-10 PMID: 3932128
-
N-Methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci.Gene. 1985;38(1-3):103-10 PMID: 3934045
-
Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.Nucleic Acids Res. 1986 Feb 25;14(4):1565-81 PMID: 3005976
-
Transcription termination and the regulation of gene expression.Annu Rev Biochem. 1986;55:339-72 PMID: 3527045
-
Expression of a Streptomyces plasmid promoter in Escherichia coli.Gene. 1986;43(3):295-300 PMID: 2427388
-
Activity of a Streptomyces transcriptional terminator in Escherichia coli.Nucleic Acids Res. 1987 Mar 25;15(6):2665-75 PMID: 3031607
-
Two promoters from the Streptomyces plasmid pIJ101 and their expression in Escherichia coli.Gene. 1987;51(2-3):179-86 PMID: 3297922
-
Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pIJ101.J Bacteriol. 1987 Sep;169(9):4177-83 PMID: 3040681
-
Random cloning and sequencing by the M13/dideoxynucleotide chain termination method.Methods Enzymol. 1987;155:51-93 PMID: 3323821
-
The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis.Mol Gen Genet. 1987 Aug;209(1):101-9 PMID: 17165236
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