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PMID: 1386367 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis.

The Journal of cell biology ·Vol. 118 ·No. 3 ·1992-08-00 ·Pages 595-605

Satterwhite LL, Lohka MJ, Wilson KL, Scherson TY, Cisek LJ, Corden JL, Pollard TD

Abstract

To understand how cytokinesis is regulated during mitosis, we tested cyclin-p34cdc2 for myosin-II kinase activity, and investigated the mitotic-specific phosphorylation of myosin-II in lysates of Xenopus eggs. Purified cyclin-p34cdc2 phosphorylated the regulatory light chain of cytoplasmic and smooth muscle myosin-II in vitro on serine-1 or serine-2 and threonine-9, sites known to inhibit the actin-activated myosin ATPase activity of smooth muscle and nonmuscle myosin (Nishikawa, M., J. R. Sellers, R. S. Adelstein, and H. Hidaka. 1984. J. Biol. Chem. 259:8808-8814; Bengur, A. R., A. E. Robinson, E. Appella, and J. R. Sellers. 1987. J. Biol. Chem. 262:7613-7617; Ikebe, M., and S. Reardon. 1990. Biochemistry. 29:2713-2720). Serine-1 or -2 of the regulatory light chain of Xenopus cytoplasmic myosin-II was also phosphorylated in Xenopus egg lysates stabilized in metaphase, but not in interphase. Inhibition of myosin-II by cyclin-p34cdc2 during prophase and metaphase could delay cytokinesis until chromosome segregation is initiated and thus determine the timing of cytokinesis relative to earlier events in mitosis.

MeSH Terms
Amino Acid Sequence Animals CDC2 Protein Kinase/metabolism Cell Cycle Cell Division Cyclins/metabolism Electrophoresis, Polyacrylamide Gel Maturation-Promoting Factor/metabolism Molecular Sequence Data Myosins/antagonists & inhibitors,metabolism Phosphorylation Substrate Specificity Time Factors Xenopus
Chemicals
Cyclins CDC2 Protein Kinase Maturation-Promoting Factor Myosins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Satterwhite L L
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Lohka M J
Wilson K L
Scherson T Y
Cisek L J
Corden J L
Pollard T D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.118.3.595
Published
1992-08-00
Pages
595-605
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289554
Subset
IM
Grants
NIGMS NIH HHS · GM-26132 · United States
External Links
PubMed source DOI: 10.1083/jcb.118.3.595 PMC Full Text View journal details
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