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PMID: 1385387 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of multiple RNA polymerase sigma factor homologs in the cyanobacterium Anabaena sp. strain PCC 7120: cloning, expression, and inactivation of the sigB and sigC genes.

Journal of bacteriology ·Vol. 174 ·No. 22 ·1992-11-00 ·Pages 7273-82

Brahamsha B, Haselkorn R

Abstract

The sigA gene of Anabaena sp. strain PCC 7120, encoding the principal RNA polymerase sigma factor, and the complement of the rpoD oligonucleotide (K. Tanaka, T. Shiina, and H. Takahashi, Science 242:1040-1042, 1988) were used as probes to isolate two genes, sigB and sigC, which encode two putative sigma factors exhibiting high degrees of similarity to SigA, to HrdA, -B, -C, and -D of Streptomyces coelicolor, and to KatF of Escherichia coli. sigB and sigC code for polypeptides of 332 and 416 amino acids with predicted molecular weights of 38,431 and 47,459, respectively. sigB and sigC mRNAs are detectable only under nitrogen-limiting conditions. Insertional inactivation of sigB and sigC indicates that neither gene alone is essential for nitrogen fixation or heterocyst differentiation.

Related Genes
hrdB sigA sigB sigC
MeSH Terms
Amino Acid Sequence Anabaena/enzymology,genetics Base Sequence Blotting, Northern Cloning, Molecular DNA-Directed RNA Polymerases/genetics,metabolism Gene Expression Gene Library Genes, Bacterial Molecular Sequence Data Mutagenesis, Insertional Oligonucleotide Probes Promoter Regions, Genetic RNA, Bacterial/genetics,isolation & purification Sequence Homology, Amino Acid Sigma Factor/genetics,metabolism Streptomyces/enzymology,genetics
Chemicals
Oligonucleotide Probes RNA, Bacterial Sigma Factor RNA polymerase sigma A DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brahamsha B
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Haselkorn R
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.22.7273-7282.1992
Published
1992-11-00
Pages
7273-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207421
Subset
IM
Grants
NIGMS NIH HHS · GM-21823 · United States
NICHD NIH HHS · HD-07136 · United States
Databases
GENBANK
L01135, L01136, L01137, L01138, L01139, L01140, L01141, M95759, M95760, Z11768
External Links
PubMed source DOI: 10.1128/jb.174.22.7273-7282.1992 PMC Full Text View journal details
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