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PMID: 1379731 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a mammalian gene structurally and functionally related to the CDC25 gene of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 7100-4

Wei W, Mosteller RD, Sanyal P, Gonzales E, McKinney D, Dasgupta C, Li P, Liu BX, Broek D

Abstract

The yeast Saccharomyces cerevisiae CDC25 gene encodes a nucleotide-exchange-factor (NEF) that can convert the inactive GDP-bound state of RAS proteins to an active RAS-GTP complex. CDC25 can activate the yeast RAS proteins as well as the human H-ras protein. CDC25 is a member of a family of yeast genes that likely encode NEFs capable of regulating the RAS-related proteins found in yeast. By aligning the amino acid sequence of CDC25-related gene products we found a number of conserved motifs. Using degenerate oligonucleotides that encode these conserved sequences, we have used polymerase chain reactions to amplify fragments of mouse and human cDNAs related to the yeast CDC25 gene. We show that a chimeric molecule, part mouse and part yeast CDC25, can suppress the loss of CDC25 function in the yeast S. cerevisiae.

Related Genes
CDC25 H-ras
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Brain/physiology Cell Cycle Proteins Chimera DNA/genetics,isolation & purification Fungal Proteins/genetics Genes Genes, Fungal Genes, ras Humans Mice Molecular Sequence Data Oligodeoxyribonucleotides Plasmids Polymerase Chain Reaction/methods RNA/genetics,isolation & purification RNA-Directed DNA Polymerase Restriction Mapping Saccharomyces cerevisiae/genetics,growth & development Sequence Homology, Nucleic Acid Temperature ras-GRF1
Chemicals
Cell Cycle Proteins Fungal Proteins Oligodeoxyribonucleotides ras-GRF1 RNA DNA RNA-Directed DNA Polymerase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Wei W
Kenneth Norris Jr. Cancer Research Institute, Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033-0800.
Mosteller R D
Sanyal P
Gonzales E
McKinney D
Dasgupta C
Li P
Liu B X
Broek D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.7100
Published
1992-08-01
Pages
7100-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49653
Subset
IM
Grants
NCI NIH HHS · CA50261 · United States
NIA NIH HHS · P05-AG05142 · United States
Databases
GENBANK
M91815
External Links
PubMed source DOI: 10.1073/pnas.89.15.7100 PMC Full Text View journal details
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