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PMID: 1373383 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

BTG1, a member of a new family of antiproliferative genes.

The EMBO journal ·Vol. 11 ·No. 4 ·1992-04-00 ·Pages 1663-70

Rouault JP, Rimokh R, Tessa C, Paranhos G, Ffrench M, Duret L, Garoccio M, Germain D, Samarut J, Magaud JP

Abstract

The BTG1 gene locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia. We report here the cloning and sequencing of the human BTG1 cDNA and establish the genomic organization of this gene. The full-length cDNA isolated from a lymphoblastoid cell line contains an open reading frame of 171 amino acids. BTG1 expression is maximal in the G0/G1 phases of the cell cycle and is down-regulated when cells progress throughout G1. Furthermore, transfection experiments of NIH3T3 cells indicate that BTG1 negatively regulates cell proliferation. The BTG1 open reading frame is 60% homologous to PC3, an immediate early gene induced by nerve growth factor in rat PC12 cells. Sequence and Northern blot analyses indicate that BTG1 and PC3 are not cognate genes. We then postulate that these two genes are the first members of a new family of antiproliferative genes.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence Cell Cycle Cell Division/genetics Chromosomes, Human, Pair 12 Chromosomes, Human, Pair 8 Cloning, Molecular DNA/genetics,isolation & purification DNA Probes Gene Library Humans Leukemia, Lymphocytic, Chronic, B-Cell/genetics Lymphocytes/physiology Mice Molecular Sequence Data Multigene Family Neoplasm Proteins/genetics RNA/genetics,isolation & purification Restriction Mapping Sequence Homology, Nucleic Acid Transfection Translocation, Genetic
Chemicals
Btg1 protein, mouse Btg1 protein, rat DNA Probes Neoplasm Proteins BTG1 protein, human RNA DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rouault J P
Equipe de Cytologie Analytique et de Cytogénétique Moléculaire Hôpital Edouard Herriot, Lyon, France.
Rimokh R
Tessa C
Paranhos G
Ffrench M
Duret L
Garoccio M
Germain D
Samarut J
Magaud J P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-04-00
Pages
1663-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556617
Subset
IM
Databases
GENBANK
X61123, X67718, X67719, X67720, X67721, X67722, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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