Abstract
Microautophagy is a versatile process in which vacuolar or lysosomal membranes directly sequester cytosolic targets for degradation. Recent genetic evidence suggested that microautophagy uses molecular machineries essential for macroautophagy, but the details of this process are still unknown. In this study, a ubiquitin-like protein Paz2 essential for micropexophagy in the yeast Pichia pastoris has been shown to receive modification through the function of Paz8 and Gsa7, yielding a modified form Paz2-I, similar to the ubiquitin-like lipidation of Aut7 that is essential for macroautophagy in Saccharomyces cerevisiae. We identified a novel membrane structure formed after the onset of micropexophagy, which we suggest is necessary for the sequestration of peroxisomes by the vacuole. Assembly of this newly formed membrane structure, which is followed by localization of Paz2 to it, was found to require a properly functioning Paz2-modification system. We herein show that Paz2 and its modification system conduct micropexophagy through formation of the membrane structure, which explains the convergence between micropexophagy and macroautophagy with regard to de novo membrane formation.
MeSH Terms
Amino Acid Sequence
Autophagy-Related Protein 8 Family
Cell Compartmentation/physiology
Cloning, Molecular
Fungal Proteins/metabolism
Intracellular Membranes/metabolism,ultrastructure
Microscopy, Electron
Microscopy, Fluorescence
Microtubule-Associated Proteins/genetics
Models, Molecular
Molecular Sequence Data
Peroxisomes/metabolism,ultrastructure
Pichia/cytology,metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Subcellular Fractions
Vacuoles/metabolism,ultrastructure
Chemicals
ATG8 protein, S cerevisiae
Autophagy-Related Protein 8 Family
Fungal Proteins
Microtubule-Associated Proteins
Saccharomyces cerevisiae Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mukaiyama Hiroyuki
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan.
Baba Misuzu
Osumi Masako
Aoyagi Satoshi
Kato Nobuo
Ohsumi Yoshinori
Sakai Yasuyoshi
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