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PMID: 12529432 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 14 ·No. 1 ·2003-01-00 ·Pages 129-41

Roberts P, Moshitch-Moshkovitz S, Kvam E, O'Toole E, Winey M, Goldfarb DS

Abstract

Nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae are formed through specific interactions between Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope. Herein, we report that NV junctions in yeast promote piecemeal microautophagy of the nucleus (PMN). During PMN, teardrop-like blebs are pinched from the nucleus, released into the vacuole lumen, and degraded by soluble hydrolases. PMN occurs in rapidly dividing cells but is induced to higher levels by carbon and nitrogen starvation and is under the control of the Tor kinase nutrient-sensing pathway. Confocal and biochemical assays demonstrate that Nvj1p is degraded in a PMN-dependent manner. PMN occurs normally in apg7-delta cells and is, therefore, not dependent on macroautophagy. Transmission electron microscopy reveals that portions of the granular nucleolus are often sequestered into PMN structures. These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole.

MeSH Terms
Autophagy-Related Protein 7 Cell Nucleus/metabolism Hydrolases/metabolism Lipoproteins/metabolism Membrane Proteins/metabolism Nerve Tissue Proteins/metabolism Nuclear Envelope/metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism Vacuoles/metabolism Vesicular Transport Proteins
Chemicals
ATG7 protein, S cerevisiae Lipoproteins Membrane Proteins Nerve Tissue Proteins Saccharomyces cerevisiae Proteins VAC8 protein, S cerevisiae Vesicular Transport Proteins Hydrolases Autophagy-Related Protein 7
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Roberts Paul
Department of Biology, University of Rochester, Rochester, New York 14627, USA.
Moshitch-Moshkovitz Sharon
Kvam Erik
O'Toole Eileen
Winey Mark
Goldfarb David S
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e02-08-0483
Published
2003-01-00
Pages
129-41
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC140233
Subset
IM
Grants
NIGMS NIH HHS · GM-59992 · United States
NIGMS NIH HHS · 5R01 GM362-11 · United States
NCRR NIH HHS · P41 RR000592 · United States
NIGMS NIH HHS · R01 GM059992 · United States
NCRR NIH HHS · RR-00592 · United States
External Links
PubMed source DOI: 10.1091/mbc.e02-08-0483 PMC Full Text View journal details
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