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PMID: 1359544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NMR structure determination reveals that the homeodomain is connected through a flexible linker to the main body in the Drosophila Antennapedia protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 22 ·1992-11-15 ·Pages 10738-42

Qian YQ, Otting G, Furukubo-Tokunaga K, Affolter M, Gehring WJ, Wüthrich K

Abstract

The secondary structure of an N-terminally elongated Antennapedia (Antp) homeodomain (HD) polypeptide containing residues -14 to 67, where residues 1-60 constitute the HD, has been determined by NMR in solution. This polypeptide contains the conserved motif -Tyr-Pro-Trp-Met- (YPWM) at positions -9 to -6. Despite the hydrophobic nature of this tetrapeptide motif, the N-terminal arm consisting of residues -14 to 6 is flexibly disordered, and the well-defined part of the HD structure with residues 7-59 is indistinguishable from that of the shorter Antp HD polypeptide (where positions 0, 1, and 67 are methionine, arginine, and glycine, respectively). In vitro biochemical studies showed that the stability and specificity of the DNA binding previously observed for the shorter Antp HD polypeptide is preserved in the elongated polypeptide. These results strongly support the view that the HD is connected through a flexible linker to the main body in the Antp protein and that the minor groove contacts by the N-terminal arm (residues 1-6) in the Antp HD-DNA complex are an intrinsic feature of the DNA-binding interactions of the intact Antp protein.

Related Genes
Antp Dfd Scr Ubx abd-A cad ems lab pb
MeSH Terms
Amino Acid Sequence Animals Antennapedia Homeodomain Protein Base Sequence Binding Sites Cloning, Molecular DNA/chemistry,metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Drosophila/genetics,metabolism Drosophila Proteins Genes, Homeobox Homeodomain Proteins Magnetic Resonance Spectroscopy/methods Molecular Sequence Data Nuclear Proteins Oligodeoxyribonucleotides Polymerase Chain Reaction/methods Protons Recombinant Proteins/chemistry,metabolism Transcription Factors
Chemicals
Antennapedia Homeodomain Protein Antp protein, Drosophila DNA-Binding Proteins Drosophila Proteins Homeodomain Proteins Nuclear Proteins Oligodeoxyribonucleotides Protons Recombinant Proteins Transcription Factors DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Qian Y Q
Institut für Molekularbiologie und Biophysik, Eidgenössiche Technische Hochschule-Hönggerberg, Zürich, Switzerland.
Otting G
Furukubo-Tokunaga K
Affolter M
Gehring W J
Wüthrich K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.22.10738
Published
1992-11-15
Pages
10738-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50417
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.22.10738 PMC Full Text View journal details
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