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PMID: 1971945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA binding properties of the purified Antennapedia homeodomain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 11 ·1990-06-00 ·Pages 4093-7

Affolter M, Percival-Smith A, Müller M, Leupin W, Gehring WJ

Abstract

The in vitro DNA binding properties of a purified 68-amino acid Antennapedia homeodomain (Antp HD) peptide have been analyzed. Equilibrium and kinetic binding studies showed that stable DNA-protein complexes are formed with a Kd of 1.6 x 10(-9) M and 1.8 x 10(-10) M, respectively. Heterodimer analysis led to the conclusion that Antp HD interacts in vitro as a monomer with the DNA target sites used in our study. The results of methylation and ethylation interference studies indicated that the Antp HD closely approaches the target DNA primarily from one side in a region extending across three phosphate backbones. The DNA binding properties of the Antp HD and prokaryotic DNA binding domains that share a helix-turn-helix motif are compared.

MeSH Terms
Alkylation Animals Antennapedia Homeodomain Protein Base Sequence Binding, Competitive DNA/metabolism DNA-Binding Proteins/metabolism Drosophila Proteins Drosophila melanogaster/genetics Genes, Homeobox Homeodomain Proteins In Vitro Techniques Kinetics Methylation Molecular Sequence Data Nuclear Proteins Peptides/metabolism Transcription Factors
Chemicals
Antennapedia Homeodomain Protein Antp protein, Drosophila DNA-Binding Proteins Drosophila Proteins Homeodomain Proteins Nuclear Proteins Peptides Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Affolter M
Biozentrum, University of Basel, Switzerland.
Percival-Smith A
Müller M
Leupin W
Gehring W J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.11.4093
Published
1990-06-00
Pages
4093-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54053
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.11.4093 PMC Full Text View journal details
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