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PMID: 1350583 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical map of Campylobacter jejuni TGH9011 and localization of 10 genetic markers by use of pulsed-field gel electrophoresis.

Journal of bacteriology ·Vol. 174 ·No. 11 ·1992-06-00 ·Pages 3494-8

Kim NW, Bingham H, Khawaja R, Louie H, Hani E, Neote K, Chan VL

Abstract

The physical map of Campylobacter jejuni TGH9011 (ATCC 43430) was constructed by mapping the three restriction enzyme sites SacII (CCGCGG), SalI (GTCGAC), and SmaI (CCCGGG) on the genome of C. jejuni by using pulsed-field gel electrophoresis and Southern hybridization. A total of 25 restriction enzyme sites were mapped onto the C. jejuni chromosome. The size of the genome was reevaluated and was shown to be 1,812.5 kb. Ten C. jejuni genetic markers that have been isolated in our laboratory were mapped to specific restriction enzyme fragments. Furthermore, we have accurately mapped one of the three rRNA operons (rrnA) and have demonstrated a separation of the 16S and 23S rRNA-encoding sequences in one of the rRNA operons.

MeSH Terms
Campylobacter jejuni/genetics Chromosome Mapping Chromosomes, Bacterial Deoxyribonucleases, Type II Site-Specific/metabolism Electrophoresis, Gel, Pulsed-Field Genetic Markers Genome, Bacterial Operon/genetics Polymorphism, Restriction Fragment Length RNA, Ribosomal/genetics Restriction Mapping
Chemicals
Genetic Markers RNA, Ribosomal CCCGGG-specific type II deoxyribonucleases CCGCGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific GTCGAC-specific type II deoxyribonucleases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kim N W
Department of Microbiology, University of Toronto, Ontario, Canada.
Bingham H
Khawaja R
Louie H
Hani E
Neote K
Chan V L
References (21)
21 references, click to expand
  1. Physical mapping of large DNA by chromosome fragmentation.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6027-31 PMID: 3045811
  2. Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis.
    J Bacteriol. 1988 Sep;170(9):4402-5 PMID: 2842319
  3. Pathophysiology of Campylobacter enteritis.
    Microbiol Rev. 1986 Mar;50(1):81-94 PMID: 3515146
  4. Nucleic acids in the classification of Campylobacters.
    Eur J Clin Microbiol. 1983 Aug;2(4):367-77 PMID: 6194994
  5. Lysyl-tRNA synthetase gene of Campylobacter jejuni.
    J Bacteriol. 1992 Feb;174(3):695-701 PMID: 1732205
  6. The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO.
    Nucleic Acids Res. 1991 Jun 25;19(12):3199-206 PMID: 1905802
  7. Complete sequence of the Campylobacter jejuni glyA gene encoding serine hydroxymethyltransferase.
    Gene. 1991 May 15;101(1):51-8 PMID: 2060796
  8. Size and physical map of the Campylobacter jejuni chromosome.
    Nucleic Acids Res. 1990 Nov 11;18(21):6211-4 PMID: 2243769
  9. Natural transformation in Campylobacter species.
    J Bacteriol. 1990 Feb;172(2):949-55 PMID: 2404960
  10. Novel arrangement of rRNA genes in Mycoplasma gallisepticum: separation of the 16S gene of one set from the 23S and 5S genes.
    J Bacteriol. 1989 May;171(5):2876-8 PMID: 2708324
  11. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
    Nucleic Acids Res. 1987 Oct 12;15(19):7865-76 PMID: 2959907
  12. Cytotoxic and cytotonic factors produced by Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis.
    J Clin Microbiol. 1986 Aug;24(2):275-81 PMID: 3018039
  13. Cloning and expression of the Campylobacter jejuni glyA gene in Escherichia coli.
    Gene. 1988 Dec 15;73(1):185-91 PMID: 3072255
  14. A physical map of the Escherichia coli K12 genome.
    Science. 1987 Jun 12;236(4807):1448-53 PMID: 3296194
  15. An electrophoretic karyotype for yeast.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756-60 PMID: 3889913
  16. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.
    Bacteriol Rev. 1973 Dec;37(4):562-603 PMID: 4203396
  17. Sizing and mapping of the genome of Campylobacter coli strain UA417R using pulsed-field gel electrophoresis.
    Gene. 1991 May 15;101(1):117-20 PMID: 1676383
  18. Physical map of the Brucella melitensis 16 M chromosome.
    J Bacteriol. 1991 Apr;173(7):2219-24 PMID: 2007548
  19. Use of pulsed-field agarose gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580.
    J Bacteriol. 1990 Sep;172(9):5211-7 PMID: 2168376
  20. A physical genome map of Pseudomonas aeruginosa PAO.
    EMBO J. 1989 Dec 20;8(13):4081-9 PMID: 2512121
  21. An unusual rRNA operon constellation: in Thermus thermophilus HB8 the 23S/5S rRNA operon is a separate entity from the 16S rRNA operon.
    Biochimie. 1987 Oct;69(10):1097-104 PMID: 3126827
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.11.3494-3498.1992
Published
1992-06-00
Pages
3494-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206033
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.11.3494-3498.1992 PMC Full Text View journal details
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