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PMID: 2708324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Novel arrangement of rRNA genes in Mycoplasma gallisepticum: separation of the 16S gene of one set from the 23S and 5S genes.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2876-8

Chen X, Finch LR

Abstract

Large restriction fragments from the DNA of Mycoplasma gallisepticum S6 and PG31, which were prepared by digestion with BglI, BssHII, SmaI, or XhoI and which were separated by pulsed-field electrophoresis, were hybridized with probes containing most, or different parts, of an rRNA operon of Mycoplasma capricolum. The results showed that the genomes contained three widely separated rRNA loci. One locus contained genes for all three rRNA species and another contained 23S and probably 5S rRNA genes, whereas the third appeared to have only a 16S rRNA gene.

MeSH Terms
Blotting, Southern DNA, Bacterial/genetics DNA, Ribosomal/genetics Mycoplasma/genetics RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Restriction Mapping
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Ribosomal RNA, Ribosomal, 16S RNA, Ribosomal, 23S RNA, Ribosomal, 5S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen X
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Finch L R
References (11)
11 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2876-2878.1989
Published
1989-05-00
Pages
2876-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209978
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.5.2876-2878.1989 PMC Full Text View journal details
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