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PMID: 1348860 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification of a mammalian 10-kDa heat shock protein, a mitochondrial chaperonin 10 homologue essential for assisted folding of trimeric ornithine transcarbamoylase in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 8 ·1992-04-15 ·Pages 3394-8

Hartman DJ, Hoogenraad NJ, Condron R, Høj PB

Abstract

We have identified a 10-kDa stress-inducible mitochondrial protein. The protein is synthesized at elevated rates in cultured rat hepatoma cells challenged with heat shock or amino acid analogues and, therefore, designated heat shock protein 10 (Hsp10). Hsp10 was purified to homogeneity from rat liver and found to exhibit a native molecular mass of 65 kDa, as opposed to a monomeric molecular mass of 10,813.4 +/- 0.41 Da. The amino acid sequence of rat Hsp10 disclosed extensive sequence similarity with bacterial chaperonin (Cpn) 10. Rat Hsp10 and Escherichia coli Cpn60 were used to reconstitute functional trimeric rat ornithine transcarbamoylase from a chemically denatured state with high efficiency. This process depended completely upon rat Hsp10 and was abolished in the presence of a nonhydrolyzable ATP analogue. We conclude that Hsp10 is a eukaryotic Cpn10 homologue and, therefore, together with Cpn60 essential for mitochondrial protein biogenesis. The Cpn-mediated protein-folding apparatus, thus, exhibits a high degree of conservation between prokaryotes and mitochondria of higher eukaryotes.

MeSH Terms
Amino Acid Sequence Animals Bacteria/genetics Bacterial Proteins/genetics Chaperonin 10 Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics,metabolism Heat-Shock Proteins/biosynthesis,genetics,isolation & purification Liver Neoplasms, Experimental/metabolism Macromolecular Substances Methionine/metabolism Mitochondria/metabolism Mitochondria, Liver/metabolism Molecular Sequence Data Molecular Weight Ornithine Carbamoyltransferase/chemistry,metabolism Protein Conformation Protein Denaturation Rats Sequence Homology, Nucleic Acid Tumor Cells, Cultured
Chemicals
Bacterial Proteins Chaperonin 10 Heat-Shock Proteins Macromolecular Substances Methionine Ornithine Carbamoyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hartman D J
Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
Hoogenraad N J
Condron R
Høj P B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.8.3394
Published
1992-04-15
Pages
3394-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48874
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.8.3394 PMC Full Text View journal details
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