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PMID: 1347668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immune response to a murine coronavirus: identification of a homing receptor-negative CD4+ T cell subset that responds to viral glycoproteins.

Virology ·Vol. 187 ·No. 2 ·1992-04-00 ·Pages 443-52

Mobley J, Evans G, Dailey MO, Perlman S

Abstract

The lymphocyte proliferative response to mouse hepatitis virus, strain JHM (MHV-JHM), a well-described cause of chronic and acute neurological infections, has been studied using vaccinia virus recombinants expressing individual MHV proteins. The surface (S) and transmembrane (M) glycoproteins were the most active proteins in causing proliferation of lymphocytes isolated from immunized adult mice, whereas lymphocytes from persistently infected mice proliferated only in response to the S protein. The cells from immunized mice which proliferated most actively in response to MHV were positive for the CD4 antigen and secreted interferon-gamma. In addition, the most responsive subset of cells did not express gp90MEL-14, the lymph node-specific homing receptor. The results identify a subpopulation of CD4+ T cells that may be an important component of the cell-mediated immune response to this virus. The data also suggest that response to the M protein is important in preventing disease progression in C57BL/6 mice since cells which recognize this protein are absent from persistently infected mice.

MeSH Terms
Animals Antigens, Viral/genetics,immunology Base Sequence CD4-Positive T-Lymphocytes/microbiology Capsid/genetics,immunology Flow Cytometry Interferon-gamma/biosynthesis Lymphocyte Activation Membrane Glycoproteins/immunology Mice Mice, Inbred C57BL Molecular Sequence Data Murine hepatitis virus/immunology Mutagenesis, Site-Directed Oligodeoxyribonucleotides/chemistry Receptors, Lymphocyte Homing/analysis Recombinant Proteins/immunology T-Lymphocyte Subsets/microbiology Viral Core Proteins/genetics,immunology Viral Envelope Proteins/genetics,immunology Viral Matrix Proteins/genetics,immunology Viral Proteins/immunology
Chemicals
Antigens, Viral Membrane Glycoproteins Oligodeoxyribonucleotides Receptors, Lymphocyte Homing Recombinant Proteins Viral Core Proteins Viral Envelope Proteins Viral Matrix Proteins Viral Proteins Interferon-gamma
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mobley J
Department of Microbiology, University of Iowa, Iowa City 52242.
Evans G
Dailey M O
Perlman S
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/0042-6822(92)90446-v
Published
1992-04-00
Pages
443-52
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7130592
Subset
IM
Grants
NIAID NIH HHS · AI22730 · United States
NINDS NIH HHS · NS01369 · United States
NINDS NIH HHS · NS24401 · United States
External Links
PubMed source DOI: 10.1016/0042-6822(92)90446-v PMC Full Text View journal details
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