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PMID: 6308569 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein.

Nucleic acids research ·Vol. 11 ·No. 15 ·1983-08-11 ·Pages 5045-54

Skinner MA, Siddell SG

Abstract

A DNA copy of the mRNA that encodes the nucleocapsid protein of Mouse Hepatitis Virus JHM has been cloned into pAT153. The DNA copy specifically inhibited the synthesis in vitro of the nucleocapsid protein. The cDNA was subcloned into M13 vectors and the entire sequence, 1767 bases including a 15 base terminal poly (A) tract, has been determined by chain-terminator sequencing. The sequence contained an open-reading frame that could encode a basic protein of mol.wt. 49700. From the predicted sequence it was apparent that the nucleocapsid protein has 5 basic regions, two of which are located near the middle of the sequence, a serine-rich region was also located, a feature which may be of functional importance as the nucleocapsid protein is phosphorylated at serine residues. The carboxy terminus of the nucleocapsid protein was found to be acidic. The 5' non-coding sequence contained a triple repeat of the pentamer AATCT, a structural feature which may play a significant role during the production of subgenomic viral mRNAs.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Capsid/genetics Cloning, Molecular DNA/analysis DNA Restriction Enzymes Murine hepatitis virus/genetics Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Rabbits Reticulocytes/metabolism Viral Proteins/genetics
Chemicals
RNA, Messenger Viral Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Skinner M A
Siddell S G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.15.5045
Published
1983-08-11
Pages
5045-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326236
Subset
IM
Databases
GENBANK
K00757
External Links
PubMed source DOI: 10.1093/nar/11.15.5045 PMC Full Text View journal details
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