Amino acid composition and electrophoretic properties of hypertensin I.

The Journal of experimental medicine ·Vol. 102 ·No. 4 ·1955-10-01 ·Pages 435-40

SKEGGS LT, MARSH WH, KAHN JR, SHUMWAY NP

Abstract

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.

Keywords

AMINO ACIDS/determination ANGIOTONIN

MeSH Terms

Amino Acids/analysis Angiotensin Amide Angiotensins Arginine Aspartic Acid Glycine Histidine Isoleucine Leucine Phenylalanine Proline Serine Tyrosine Valine

Chemicals

Amino Acids Angiotensins Isoleucine Aspartic Acid Tyrosine Serine Phenylalanine Histidine Angiotensin Amide Arginine Proline Leucine Valine Glycine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

SKEGGS L T

MARSH W H

KAHN J R

SHUMWAY N P

References (7)

7 references, click to expand

The assay of hypertensin from the arterial blood of normotensive and hypertensive human beings.
J Exp Med. 1952 Jun;95(6):523-9 PMID: 14946317
The purification of hypertensin I.
J Exp Med. 1954 Oct 1;100(4):363-70 PMID: 13201713
A method of assaying small amounts of hypertensin.
Lab Invest. 1953 Mar-Apr;2(2):109-14 PMID: 13045413
The existence of two forms of hypertensin.
J Exp Med. 1954 Mar;99(3):275-82 PMID: 13130799
Electrophoretic properties of oxytocin.
J Biol Chem. 1953 Feb;200(2):559-64 PMID: 13034814
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Chromatography of amino acids on sulfonated polystyrene resins.
J Biol Chem. 1951 Oct;192(2):663-81 PMID: 14907661

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.102.4.435

Published

1955-10-01

Pages

435-40

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2136516

Subset

External Links

PubMed source DOI: 10.1084/jem.102.4.435 PMC Full Text View journal details

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Amino acid composition and electrophoretic properties of hypertensin I.

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