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PMID: 1324416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ABF1 is a phosphoprotein and plays a role in carbon source control of COX6 transcription in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 12 ·No. 9 ·1992-09-00 ·Pages 4197-208

Silve S, Rhode PR, Coll B, Campbell J, Poyton RO

Abstract

Previously, we have shown that the Saccharomyces cerevisiae DNA-binding protein ABF1 exists in at least two different electrophoretic forms (K. S. Sweder, P. R. Rhode, and J. L. Campbell, J. Biol. Chem. 263: 17270-17277, 1988). In this report, we show that these forms represent different states of phosphorylation of ABF1 and that at least four different phosphorylation states can be resolved electrophoretically. The ratios of these states to one another differ according to growth conditions and carbon source. Phosphorylation of ABF1 is therefore a regulated process. In nitrogen-starved cells or in cells grown on nonfermentable carbon sources (e.g., lactate), phosphorylated forms predominate, while in cells grown on fermentable carbon sources (e.g., glucose), dephosphorylated forms are enriched. The phosphorylation pattern is affected by mutations in the SNF1-SSN6 pathway, which is involved in glucose repression-depression. Whereas a functional SNF1 gene, which encodes a protein kinase, is not required for the phosphorylation of ABF1, a functional SSN6 gene is required for itsd ephosphorylation. The phosphorylation patterns that we have observed correlate with the regulation of a specific target gene, COX6, which encodes subunit VI of cytochrome c oxidase. Transcription of COX6 is repressed by growth in medium containing a fermentable carbon source and is derepressed by growth in medium containing a nonfermentable carbon source. COX6 repression-derepression is under the control of the SNF1-SSN6 pathway. This carbon source regulation is exerted through domain 1, a region of the upstream activation sequence UAS6 that binds ABF1 (J. D. Trawick, N. Kraut, F. Simon, and R. O. Poyton, Mol. Cell Biol. 12:2302-2314, 1992). We show that the greater the phosphorylation of ABF1, the greater the transcription of COX6. Furthermore, the ABF1-containing protein-DNA complexes formed at domain 1 differ according to the phosphorylation state of ABF1 and the carbon source on which the cells were grown. From these findings, we propose that the phosphorylation of ABF1 is involved in glucose repression-derepression of COX6 transcription.

Related Genes
COX6
MeSH Terms
Base Sequence Binding Sites DNA, Fungal/metabolism DNA-Binding Proteins Electron Transport Complex IV/genetics,metabolism Fungal Proteins/metabolism Gene Expression Regulation, Fungal Molecular Sequence Data Phosphoproteins/metabolism Phosphorylation Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Transcription Factors/metabolism Transcription, Genetic
Chemicals
ABF1 protein, S cerevisiae DNA, Fungal DNA-Binding Proteins Fungal Proteins Phosphoproteins Saccharomyces cerevisiae Proteins Transcription Factors Electron Transport Complex IV
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Silve S
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
Rhode P R
Coll B
Campbell J
Poyton R O
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.9.4197-4208.1992
Published
1992-09-00
Pages
4197-208
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360325
Subset
IM
Grants
NIGMS NIH HHS · GM25508 · United States
NIGMS NIH HHS · GM30228 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.9.4197-4208.1992 PMC Full Text View journal details
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