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PMID: 1323125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Brief dendritic calcium signals initiate long-lasting synaptic depression in cerebellar Purkinje cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 7051-5

Konnerth A, Dreessen J, Augustine GJ

Abstract

We have performed experiments designed to test the hypothesis that long-term depression (LTD) of excitatory synaptic transmission in the cerebellar cortex is caused by a rise in postsynaptic Ca concentration. These experiments combined measurements of synaptic efficacy, performed with the thin slice patch clamp technique, with fura-2 measurements of intracellular Ca concentration ([Ca]i) in single cerebellar Purkinje cells. Simultaneous activation of the climbing fiber and parallel fibers innervating single Purkinje cells caused a LTD of transmission of the parallel fiber-Purkinje cell excitatory synapse. This LTD was associated with large and transient rises in [Ca]i in the Purkinje cell and apparently was due to Ca entry through voltage-gated Ca channels in the Purkinje cell dendrites. The rise in [Ca]i produced by climbing fiber activity was necessary for LTD, because addition of the Ca chelator bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate (BAPTA) to the interior of the Purkinje cell blocked LTD. Further, elevation of [Ca]i, produced by depolarizing pulses delivered in conjunction with parallel fiber activation, induced a depression of synaptic activity that closely resembled LTD in both time course and magnitude. Thus, a rise in [Ca]i appears to be sufficient to initiate LTD. From these results, we conclude that LTD of the parallel fiber-Purkinje cell synapse is initiated by a brief, climbing fiber-mediated rise in postsynaptic [Ca]i and that LTD is maintained by other, longer-lived processes that are triggered by the rise in postsynaptic [Ca]i.

MeSH Terms
Animals Calcium/metabolism Calcium Channels/drug effects,physiology Cerebellar Cortex/physiology Dendrites/physiology Egtazic Acid/analogs & derivatives,pharmacology In Vitro Techniques Kinetics Purkinje Cells/physiology Rats Synapses/physiology Synaptic Transmission Time Factors
Chemicals
Calcium Channels Egtazic Acid 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Konnerth A
Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
Dreessen J
Augustine G J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.7051
Published
1992-08-01
Pages
7051-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49643
Subset
IM
Grants
NINDS NIH HHS · NS 21624 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.15.7051 PMC Full Text View journal details
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