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PMID: 1314803 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat.

Journal of bacteriology ·Vol. 174 ·No. 9 ·1992-05-00 ·Pages 2891-7

Parent R, Roy PH

Abstract

We have sequenced the gene coding for the chloramphenicol acetyltransferase of Tn2424 of plasmid NR79. This gene codes for a protein of 23,500 Da, and the derived protein sequence is similar to those of the chromosomal chloramphenicol acetyltransferases of Agrobacterium tumefaciens and Pseudomonas aeruginosa and of unidentified open reading frames, which may encode chloramphenicol acetyltransferases, adjacent to the ermG macrolide-lincosamide-streptogramin resistance gene of Bacillus sphaericus and the vgb virginiamycin resistance gene of Staphylococcus aureus. Weaker similarity to the LacA (thiogalactoside acetyltransferase) and CysE (serine acetyltransferase) proteins of Escherichia coli and the NodL protein of Rhizobium leguminosarum is also observed. There is no significant similarity to any other chloramphenicol acetyltransferase genes, such as that of Tn9. The Tn2424 cat gene is part of a 4.5-kb region which also contains the aacA1a aminoglycoside-6'-N-acetyltransferase gene; Tn2424 is similar to Tn21 except for the presence of this region. Sequences flanking the cat gene are typical of those flanking other genes inserted into pVS1-derived "integrons" by a site-specific recombinational mechanism.

MeSH Terms
Agrobacterium tumefaciens/genetics Amino Acid Sequence Base Sequence Chloramphenicol/metabolism Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Consensus Sequence DNA Transposable Elements/genetics Escherichia coli/genetics Genes, Bacterial/genetics Molecular Sequence Data Multigene Family/genetics Plasmids/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements Chloramphenicol Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parent R
Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Sainte-Foy, Québec, Canada.
Roy P H
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.9.2891-2897.1992
Published
1992-05-00
Pages
2891-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205941
Subset
IM
Databases
GENBANK
M79367, M79368, M79369, M79370, M79371, M79372, M80188, M81469, M89479, M89480
External Links
PubMed source DOI: 10.1128/jb.174.9.2891-2897.1992 PMC Full Text View journal details
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