Home LiteratureArticle Details
PMID: 1313920 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specific interactions of HeLa cell proteins with proposed translation domains of the poliovirus 5' noncoding region.

Journal of virology ·Vol. 66 ·No. 5 ·1992-05-00 ·Pages 3101-9

Gebhard JR, Ehrenfeld E

Abstract

To determine which sequences or structures in the poliovirus 5' noncoding region (5'NCR) are involved in binding proteins used for internal ribosome binding and protein synthesis initiation, translation competition assays were performed in rabbit reticulocyte lysates in the presence and absence of HeLa cell extract. The results revealed two functional domains in the poliovirus 5'NCR. One, requiring nucleotides (nts) 457 to 626, binds proteins that are required for translation of all mRNAs and that are present in both reticulocyte lysates and HeLa cell extracts. Another, contained within nts 286 to 456, interacts with proteins that are specific for poliovirus translation and are present in HeLa cells but not in significant amounts in rabbit reticulocyte lysates. In order to detect HeLa cell proteins that interact stably with the 5'NCR of poliovirus, UV cross-linking was used. At least four major protein-RNA complexes were identified, three of which were shown by RNA competition analysis to bind specifically to defined domains within the 5'NCR. Protein A (54 kDa) cross-linked to RNA sequences and/or structures located between nts 457 and 626; proteins B (48 kDa) and C (38 kDa) bound to nts 286 to 456.

MeSH Terms
Binding, Competitive Cell Extracts Chromosome Mapping Cross-Linking Reagents DNA-Binding Proteins/metabolism HeLa Cells/chemistry,metabolism Host-Parasite Interactions Humans Models, Biological Poliomyelitis/metabolism Poliovirus/metabolism Protein Biosynthesis RNA, Viral/metabolism Reticulocytes/metabolism
Chemicals
Cell Extracts Cross-Linking Reagents DNA-Binding Proteins RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gebhard J R
Department of Cellular, Viral, and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Ehrenfeld E
References (46)
46 references, click to expand
  1. Translation of poliovirus RNA in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash.
    Virology. 1979 Sep;97(2):396-405 PMID: 224589
  2. A protein covalently linked to poliovirus genome RNA.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):59-63 PMID: 189316
  3. The novel mechanism of initiation of picornavirus RNA translation.
    Trends Biochem Sci. 1990 Dec;15(12):477-83 PMID: 2077688
  4. Gross rearrangements within the 5'-untranslated region of the picornaviral genomes.
    Nucleic Acids Res. 1990 Jun 11;18(11):3371-5 PMID: 2162521
  5. A functional ribonucleoprotein complex forms around the 5' end of poliovirus RNA.
    Cell. 1990 Oct 19;63(2):369-80 PMID: 2170027
  6. Common structures of the 5' non-coding RNA in enteroviruses and rhinoviruses. Thermodynamical stability and statistical significance.
    J Mol Biol. 1990 Dec 5;216(3):729-41 PMID: 2175364
  7. Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
    Genes Dev. 1989 Dec;3(12A):1874-86 PMID: 2533575
  8. Conserved structural domains in the 5'-untranslated region of picornaviral genomes: an analysis of the segment controlling translation and neurovirulence.
    Virology. 1989 Feb;168(2):201-9 PMID: 2536978
  9. New model for the secondary structure of the 5' non-coding RNA of poliovirus is supported by biochemical and genetic data that also show that RNA secondary structure is important in neurovirulence.
    J Mol Biol. 1989 May 20;207(2):379-92 PMID: 2547075
  10. Conservation of the secondary structure elements of the 5'-untranslated region of cardio- and aphthovirus RNAs.
    Nucleic Acids Res. 1989 Jul 25;17(14):5701-11 PMID: 2548167
  11. A cellular protein that binds to the 5'-noncoding region of poliovirus RNA: implications for internal translation initiation.
    Genes Dev. 1989 Jul;3(7):1026-34 PMID: 2550319
  12. Poliovirus translation: a paradigm for a novel initiation mechanism.
    Bioessays. 1989 Nov;11(5):128-32 PMID: 2556117
  13. Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.
    Mol Cell Biol. 1988 Mar;8(3):1103-12 PMID: 2835660
  14. Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region.
    J Virol. 1988 Jul;62(7):2219-27 PMID: 2836606
  15. Comparative sequence analysis of the 5' noncoding region of the enteroviruses and rhinoviruses.
    Virology. 1988 Jul;165(1):42-50 PMID: 2838971
  16. An internal 5'-noncoding region required for translation of poliovirus RNA in vitro.
    J Virol. 1988 Aug;62(8):3068-72 PMID: 2839722
  17. A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.
    J Virol. 1988 Aug;62(8):2636-43 PMID: 2839690
  18. Translation in mammalian cells of a gene linked to the poliovirus 5' noncoding region.
    Science. 1988 Jul 22;241(4864):445-8 PMID: 2839901
  19. Mutational analysis of upstream AUG codons of poliovirus RNA.
    J Virol. 1988 Dec;62(12):4486-92 PMID: 2846865
  20. Modulation of the expression of poliovirus proteins in reticulocyte lysates.
    Virology. 1986 Jan 30;148(2):255-67 PMID: 3002037
  21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  22. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  23. Unusual requirements for optimum translation of polio viral RNA in vitro.
    J Biol Chem. 1983 Jun 10;258(11):7195-9 PMID: 6304083
  24. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA.
    Nature. 1988 Jul 28;334(6180):320-5 PMID: 2839775
  25. Point mutations modify the response of poliovirus RNA to a translation initiation factor: a comparison of neurovirulent and attenuated strains.
    Virology. 1988 Oct;166(2):394-404 PMID: 2845653
  26. The genomes of attenuated and virulent poliovirus strains differ in their in vitro translation efficiencies.
    Virology. 1985 Dec;147(2):243-52 PMID: 3000069
  27. Phosphorylation within a specific domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro.
    Cell. 1987 May 8;49(3):407-14 PMID: 3032453
  28. Construction of viable deletion and insertion mutants of the Sabin strain of type 1 poliovirus: function of the 5' noncoding sequence in viral replication.
    J Virol. 1987 May;61(5):1478-87 PMID: 3033275
  29. Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants.
    J Mol Biol. 1984 Apr 25;174(4):561-85 PMID: 6202874
  30. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
  31. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
  32. In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate.
    J Virol. 1984 May;50(2):507-14 PMID: 6323749
  33. 5'-terminal structure of poliovirus polyribosomal RNA is pUp.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 PMID: 174102
  34. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5 PMID: 191841
  35. Internal initiation of translation mediated by the 5' leader of a cellular mRNA.
    Nature. 1991 Sep 5;353(6339):90-4 PMID: 1652694
  36. Substitutions in the protease (3Cpro) gene of poliovirus can suppress a mutation in the 5' noncoding region.
    J Virol. 1990 Feb;64(2):607-12 PMID: 2153227
  37. Oxidation-reduction sensitive interaction of a cellular 50-kDa protein with an RNA hairpin in the 5' noncoding region of the poliovirus genome.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5846-50 PMID: 2165605
  38. A region of the 5' noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control.
    J Virol. 1990 Nov;64(11):5389-95 PMID: 2170677
  39. The deletion of 41 proximal nucleotides reverts a poliovirus mutant containing a temperature-sensitive lesion in the 5' noncoding region of genomic RNA.
    J Virol. 1989 Feb;63(2):847-62 PMID: 2536107
  40. Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.
    J Virol. 1989 Apr;63(4):1651-60 PMID: 2538648
  41. Translation of glucose-regulated protein 78/immunoglobulin heavy-chain binding protein mRNA is increased in poliovirus-infected cells at a time when cap-dependent translation of cellular mRNAs is inhibited.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5795-9 PMID: 2548189
  42. Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8299-303 PMID: 2554308
  43. Distinct modes of poliovirus polyprotein initiation in vitro.
    Virus Res. 1989 Oct;14(2):107-18 PMID: 2558458
  44. An RNA sequence of hundreds of nucleotides at the 5' end of poliovirus RNA is involved in allowing viral protein synthesis.
    J Virol. 1988 Jul;62(7):2291-9 PMID: 2836612
  45. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 PMID: 174106
  46. The 5'-untranslated region of picornaviral genomes.
    Adv Virus Res. 1991;40:103-80 PMID: 1957717
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.5.3101-3109.1992
Published
1992-05-00
Pages
3101-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241072
Subset
IM
Grants
NIAID NIH HHS · AI12387 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.5.3101-3109.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2