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PMID: 1312627 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A deletion in the simian virus 40 large T antigen impairs lytic replication in monkey cells in vivo but enhances DNA replication in vitro: new complementation function of T antigen.

Journal of virology ·Vol. 66 ·No. 4 ·1992-04-00 ·Pages 2195-207

Maulbecker C, Mohr I, Gluzman Y, Bartholomew J, Botchan M

Abstract

We describe a new complementation function within the simian virus 40 (SV40) A gene. This function is required for viral DNA replication and virus production in vivo but, surprisingly, does not affect any of the intrinsic enzymatic functions of T antigen directly required for in vitro DNA replication. Other well-characterized SV40 T-antigen mutants, whether expressed stably from integrated genomes or in cotransfection experiments, complement these mutants for in vivo DNA replication and plaque formation. These new SV40 mutants were isolated and cloned from human cells which stably carry the viral DNA. The alteration in the large-T-antigen gene was shown by marker rescue and nucleotide sequence analysis to be a deletion of 322 bp spanning the splice-donor site of the first exon, creating a 14-amino-acid deletion in the large T antigen. The mutant gene was expressed in H293 human cells from an adenovirus vector, and the protein was purified by immunoaffinity chromatography. The mutant protein directs greater levels of DNA replication in vitro than does the wild-type protein. Moreover, the mutant protein reduces the lag time for in vitro DNA synthesis and can be diluted to lower levels than wild-type T antigen and still promote good replication, which is in clear contrast to the in vivo situation. These biochemical features of the protein are independent of the source of the cellular replication factors (i.e., HeLa, H293, COS 7, or CV1 cells) and the cells from which the T antigens were purified. The mutant T antigen does not transform Rat-2 cells. Several different models which might reconcile the differences observed in vivo and in vitro are outlined. We propose that the function of T antigen affected prepares cells for SV40 replication by activation of a limiting cellular replication factor. Furthermore, a link between the induction of a cellular replication factor and transformation by SV40 is discussed.

MeSH Terms
Amino Acid Sequence Animals Antigens, Polyomavirus Transforming/chemistry,physiology Cell Line Cell Transformation, Viral/genetics Chromosome Deletion Cloning, Molecular DNA Replication Exons Genetic Complementation Test Haplorhini Kinetics Molecular Sequence Data Plasmids Simian virus 40/genetics,physiology Virus Replication
Chemicals
Antigens, Polyomavirus Transforming
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Maulbecker C
Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Mohr I
Gluzman Y
Bartholomew J
Botchan M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.4.2195-2207.1992
Published
1992-04-00
Pages
2195-207
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC289012
Subset
IM
Grants
NCI NIH HHS · CA 30490 · United States
NIEHS NIH HHS · ES01896 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.4.2195-2207.1992 PMC Full Text View journal details
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