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PMID: 6092946 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Complex regulation of simian virus 40 early-region transcription from different overlapping promoters.

Molecular and cellular biology ·Vol. 4 ·No. 9 ·1984-09-00 ·Pages 1900-14

Buchman AR, Fromm M, Berg P

Abstract

During simian virus 40 lytic infection there is a shift in initiation sites used to transcribe the early region, which encodes large T and small t antigens. Early in infection, transcription is initiated almost exclusively from sites that are downstream of the origin of DNA replication, whereas transcripts produced later are initiated mainly from sites on the upstream side. We have used mutant virus and specially constructed plasmid DNAs to investigate the factors regulating this transcriptional shift. In our studies simian virus 40 large T antigen appears to mediate the shift in transcription in two ways: first, T antigen represses transcription at the downstream sites late in infection by binding to the region where these RNAs are initiated; second, T antigen promotes transcription from sites on the upstream side by its ability to initiate replication or amplification, or both, of the template DNA. In addition, transcription from the downstream sites is heavily dependent on enhancer sequences located in the 72-base-pair repeat region, whereas transcription from the upstream sites late in infection does not require enhancer sequences. Thus, different overlapping promoters regulate simian virus 40 early-region expression in a manner that apparently coordinates the production of large T antigen with the increase in viral DNA.

MeSH Terms
Animals Antigens, Viral/genetics Base Sequence Cell Line Chlorocebus aethiops DNA Restriction Enzymes Enhancer Elements, Genetic Genes Genes, Viral Kidney Plasmids Promoter Regions, Genetic Simian virus 40/genetics,immunology Transcription, Genetic Transfection
Chemicals
Antigens, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buchman A R
Fromm M
Berg P
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57 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.9.1900-1914.1984
Published
1984-09-00
Pages
1900-14
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369000
Subset
IM
Grants
NIGMS NIH HHS · 5-T32GM-07599 · United States
NCI NIH HHS · CA 15513 · United States
NIGMS NIH HHS · GM-13235 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.9.1900-1914.1984 PMC Full Text View journal details
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