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PMID: 1310765 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A 10-base-pair element of the human immunodeficiency virus type 1 long terminal repeat (LTR) is an absolute requirement for transactivation by the human cytomegalovirus 72-kilodalton IE1 protein but can be compensated for by other LTR regions in transactivation by the 80-kilodalton IE2 protein.

Journal of virology ·Vol. 66 ·No. 3 ·1992-03-00 ·Pages 1543-50

Walker S, Hagemeier C, Sissons JG, Sinclair JH

Abstract

Transient gene expression studies have indicated that human cytomegalovirus (HCMV) specifically transactivates the human immunodeficiency virus (HIV) long terminal repeat (LTR). We show here, by a specific mutational analysis, that only the TATA box region is obligatory for transactivation of the HIV-1 LTR by HCMV. Similarly, this element is also sufficient for transactivation by either the HCMV 72-kDa major immediate-early 1 (IE1) or 80-kDa IE2 gene product independently. However, deletion of a 10-bp region from the minimal responsive element, 5' to the TATA box, dramatically reduced the level of HCMV 72-kDa IE1 or 80-kDa IE2 transactivation, indicating a crucial role for this element in transactivation. Whereas inclusion of the TAR element or Sp1 sites on this 10-bp-deleted minimal promoter had no effect on the removal of IE1 transactivation, TAR and Sp1 elements did compensate for the 10-bp element in transactivation by IE2 and HCMV. Consequently, the sequence requirements of the HIV-1 LTR for transactivation by HCMV can be reproduced by these IE1 and IE2 gene products of HCMV.

Related Genes
HSP-70 c-fos c-myc hps
MeSH Terms
Base Sequence Cytomegalovirus/genetics DNA Mutational Analysis HIV Long Terminal Repeat HIV-1/genetics Immediate-Early Proteins/genetics Membrane Glycoproteins Molecular Sequence Data Nuclear Proteins/genetics Regulatory Sequences, Nucleic Acid Sequence Alignment Trans-Activators Transcriptional Activation Viral Envelope Proteins Viral Proteins/genetics
Chemicals
IE1 protein, cytomegalovirus IE2 protein, Cytomegalovirus Immediate-Early Proteins Membrane Glycoproteins Nuclear Proteins Trans-Activators UL115 protein, Human herpesvirus 5 Viral Envelope Proteins Viral Proteins glycoprotein H, Cytomegalovirus glycoprotein H, Human cytomegalovirus glycoprotein O, cytomegalovirus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Walker S
Department of Medicine, University of Cambridge, United Kingdom.
Hagemeier C
Sissons J G
Sinclair J H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.3.1543-1550.1992
Published
1992-03-00
Pages
1543-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240880
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/JVI.66.3.1543-1550.1992 PMC Full Text View journal details
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