A 24-amino-acid polypeptide is essential for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone.

Journal of bacteriology ·Vol. 174 ·No. 4 ·1992-02-00 ·Pages 1426-7

Goosen N, Huinen RG, van de Putte P

Abstract

At least four genes are required for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone (PQQ) in Acinetobacter calcoaceticus. The DNA region where one of these genes was mapped codes for a polypeptide of only 24 amino acids. Here we show that indeed this small peptide is essential for PQQ synthesis. Site-directed mutagenesis shows that at least one glutamate and one tyrosine residue of the polypeptide are essential for its function.

Related Genes

pqq

MeSH Terms

Acinetobacter calcoaceticus/genetics,metabolism Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Base Sequence Coenzymes/metabolism Molecular Sequence Data Mutagenesis, Site-Directed PQQ Cofactor Quinolones/metabolism

Chemicals

Bacterial Proteins Coenzymes Quinolones PQQ Cofactor

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Goosen N

Department of Molecular Genetics, Gorlaeus Laboratories, University of Leiden, The Netherlands.

Huinen R G

van de Putte P

References (12)

12 references, click to expand

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Existence of a novel prosthetic group, PQQ, in membrane-bound, electron transport chain-linked, primary dehydrogenases of oxidative bacteria.
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J Bacteriol. 1985 Aug;163(2):493-9 PMID: 3926746
Cloning of the genes involved in synthesis of coenzyme pyrrolo-quinoline-quinone from Acinetobacter calcoaceticus.
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L-tyrosine is the precursor of PQQ biosynthesis in Hyphomicrobium X.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.174.4.1426-1427.1992

Published

1992-02-00

Pages

1426-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC206443

Subset

External Links

PubMed source DOI: 10.1128/jb.174.4.1426-1427.1992 PMC Full Text View journal details

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A 24-amino-acid polypeptide is essential for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone.

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