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PMID: 12941889 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The cap region of topoisomerase I binds to sites near both ends of simian virus 40 T antigen.

Journal of virology ·Vol. 77 ·No. 18 ·2003-09-00 ·Pages 9809-16

Roy R, Trowbridge P, Yang Z, Champoux JJ, Simmons DT

Abstract

Two independent binding sites on simian virus 40 (SV40) T antigen for topoisomerase I (topo I) were identified. One was mapped to the N-terminal domain (residues 83 to 160) by a combination of enzyme-linked immunosorbent assays (ELISAs) and glutathione S-transferase (GST) pull-down assays performed with various T antigen deletion mutants. The second was mapped to the C-terminal domain (residues 602 to 708). The region in human topo I that binds to both sites in T antigen was identified by ELISAs, GST pull-down assays, and double-hexamer binding assays with topo I deletion mutants. This region corresponds to a distinct domain on topo I known as the cap region that maps from residues 175 to 433. By combining these data with information about the structure of T-antigen double hexamers associated with origin DNA, we propose that the cap region of topo I associates specifically with both ends of the double hexamer bound to the SV40 origin to initiate DNA replication.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/chemistry,metabolism Binding Sites DNA Replication DNA Topoisomerases, Type I/chemistry,metabolism Models, Molecular Simian virus 40/immunology
Chemicals
Antigens, Polyomavirus Transforming DNA Topoisomerases, Type I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roy Rupa
Department of Biological Sciences, University of Delaware, Newark, DE 19716-2590, USA.
Trowbridge Pamela
Yang Zheng
Champoux James J
Simmons Daniel T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.18.9809-9816.2003
Published
2003-09-00
Pages
9809-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC224608
Subset
IM
Grants
NCI NIH HHS · R01 CA036118 · United States
NIGMS NIH HHS · R01 GM060330 · United States
NCI NIH HHS · CA 36118 · United States
NIGMS NIH HHS · GM 60330 · United States
External Links
PubMed source DOI: 10.1128/jvi.77.18.9809-9816.2003 PMC Full Text View journal details
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