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PMID: 12872004 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the Drosophila U7 small nuclear RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 16 ·2003-08-05 ·Pages 9422-7

Dominski Z, Yang XC, Purdy M, Marzluff WF

Abstract

Base pairing between the 5' end of U7 small nuclear RNA (snRNA) and the histone downstream element (HDE) in replication-dependent histone pre-mRNAs is the key event in 3'-end processing that leads to generation of mature histone mRNAs. We have cloned the Drosophila U7 snRNA and demonstrated that it is required for histone pre-mRNA 3'-end processing in a Drosophila nuclear extract. The 71-nt Drosophila U7 snRNA is encoded by a single gene that is embedded in the direct orientation in an intron of the Eip63E gene. The U7 snRNA gene contains conserved promoter elements typical of other Drosophila snRNA genes, and the coding sequence is followed by a 3' box indicating that the Drosophila U7 snRNA gene is an independent transcription unit. Drosophila U7 snRNA contains a trimethyl-guanosine cap at the 5' end and a putative Sm-binding site similar to the unique Sm-binding site found in other U7 snRNAs. Drosophila U7 snRNA is approximately 10 nt longer than mammalian U7 snRNAs because of an extended 5' sequence and has only a limited potential to form a stem-loop structure near the 3' end. The extended 5' end of Drosophila U7 snRNA can base pair with the HDE in all five Drosophila histone pre-mRNAs. Blocking the 5' end of the U7 snRNA with a complementary oligonucleotide specifically blocks processing of a Drosophila histone pre-mRNA. Changes in the HDE that abolish or decrease processing efficiency result in a reduced ability to recruit U7 snRNA to the pre-mRNA.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Northern Cell Nucleus/metabolism Cloning, Molecular Drosophila Introns Mice Models, Genetic Molecular Sequence Data Precipitin Tests Promoter Regions, Genetic RNA, Messenger/metabolism RNA, Small Nuclear/chemistry,genetics
Chemicals
RNA, Messenger RNA, Small Nuclear U7 small nuclear RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dominski Zbigniew
Department of Biochemistry and Biophysics and Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA. dominski@med.unc.edu
Yang Xiao-Cui
Purdy Matthew
Marzluff William F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1533509100
Email
dominski@med.unc.edu
Published
2003-08-05
Epub
2003-00-18
Pages
9422-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC170934
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058921 · United States
NIGMS NIH HHS · GM58921 · United States
External Links
PubMed source DOI: 10.1073/pnas.1533509100 PMC Full Text View journal details
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