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PMID: 11251071 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact.

Molecular biology of the cell ·Vol. 12 ·No. 3 ·2001-03-00 ·Pages 565-76

Shopland LS, Byron M, Stein JL, Lian JB, Stein GS, Lawrence JB

Abstract

Interactions between Cajal bodies (CBs) and replication-dependent histone loci occur more frequently than for other mRNA-encoding genes, but such interactions are not seen with all alleles at a given time. Because CBs contain factors required for transcriptional regulation and 3' end processing of nonpolyadenylated replication-dependent histone transcripts, we investigated whether interaction with CBs is related to metabolism of these transcripts, known to vary during the cell cycle. Our experiments revealed that a locus containing a cell cycle-independent, replacement histone gene that produces polyadenylated transcripts does not preferentially associate with CBs. Furthermore, modest but significant changes in association levels of CBs with replication-dependent histone loci mimic their cell cycle modulations in transcription and 3' end processing rates. By simultaneously visualizing replication-dependent histone genes and their nuclear transcripts for the first time, we surprisingly find that the vast majority of loci producing detectable RNA foci do not contact CBs. These studies suggest some link between CB association and unusual features of replication-dependent histone gene expression. However, sustained CB contact is not a requirement for their expression, consistent with our observations of U7 snRNP distributions. The modest correlation to gene expression instead may reflect transient gene signaling or the nucleation of small CBs at gene loci.

MeSH Terms
Cell Division Coiled Bodies/genetics,metabolism Gene Expression Genetic Variation HeLa Cells Histones/genetics Humans In Situ Hybridization, Fluorescence RNA, Messenger/genetics,metabolism RNA, Small Nuclear/genetics,metabolism
Chemicals
Histones RNA, Messenger RNA, Small Nuclear U7 small nuclear RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shopland L S
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
Byron M
Stein J L
Lian J B
Stein G S
Lawrence J B
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.12.3.565
Published
2001-03-00
Pages
565-76
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC30964
Subset
IM
Grants
NIGMS NIH HHS · F32 GM018846 · United States
NIAMS NIH HHS · AR-42262 · United States
NIGMS NIH HHS · GM-18846 · United States
NIGMS NIH HHS · GM-49254 · United States
External Links
PubMed source DOI: 10.1091/mbc.12.3.565 PMC Full Text View journal details
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