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PMID: 12869580 Published · ppublish English Comparative Study Journal Article

Integrated mapping, chromosomal sequencing and sequence analysis of Cryptosporidium parvum.

Genome research ·Vol. 13 ·No. 8 ·2003-08-00 ·Pages 1787-99

Bankier AT, Spriggs HF, Fartmann B, Konfortov BA, Madera M, Vogel C, Teichmann SA, Ivens A, Dear PH

Abstract

The apicomplexan Cryptosporidium parvum is one of the most prevalent protozoan parasites of humans. We report the physical mapping of the genome of the Iowa isolate, sequencing and analysis of chromosome 6, and approximately 0.9 Mbp of sequence sampled from the remainder of the genome. To construct a robust physical map, we devised a novel and general strategy, enabling accurate placement of clones regardless of clone artefacts. Analysis reveals a compact genome, unusually rich in membrane proteins. As in Plasmodium falciparum, the mean size of the predicted proteins is larger than that in other sequenced eukaryotes. We find several predicted proteins of interest as potential therapeutic targets, including one exhibiting similarity to the chloroquine resistance protein of Plasmodium. Coding sequence analysis argues against the conventional phylogenetic position of Cryptosporidium and supports an earlier suggestion that this genus arose from an early branching within the Apicomplexa. In agreement with this, we find no significant synteny and surprisingly little protein similarity with Plasmodium. Finally, we find two unusual and abundant repeats throughout the genome. Among sequenced genomes, one motif is abundant only in C. parvum, whereas the other is shared with (but has previously gone unnoticed in) all known genomes of the Coccidia and Haemosporida. These motifs appear to be unique in their structure, distribution and sequences.

MeSH Terms
Animals Base Composition/genetics Centromere/genetics Cryptosporidiosis/diagnosis,microbiology,therapy Cryptosporidium parvum/genetics,isolation & purification,pathogenicity DNA, Protozoan/analysis Gene Dosage Genetic Therapy Genome, Protozoan Molecular Sequence Data Phylogeny Physical Chromosome Mapping/methods Polymorphism, Genetic/genetics Polymorphism, Single Nucleotide/genetics Sequence Analysis, DNA/methods Tandem Repeat Sequences/genetics Telomere/genetics
Chemicals
DNA, Protozoan
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Bankier Alan T
Medical Research Council (MRC) Laboratory of Molecular Biology, Cambridge CB 2 2QH, UK.
Spriggs Helen F
Fartmann Berthold
Konfortov Bernard A
Madera Martin
Vogel Christine
Teichmann Sarah A
Ivens Al
Dear Paul H
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.1555203
Published
2003-08-00
Epub
2003-00-17
Pages
1787-99
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC403770
Subset
IM
Databases
GENBANK
AJ561222, AJ561223, AJ561224, AJ561225, AJ561226, AJ561227, AJ561228, AJ561229, AJ561230, AJ561231, AJ561232, AJ561233, AJ561234, AJ561235, AJ561236, AJ561237, AJ561238, AJ561239, AJ561240, AJ561241, AJ561242, AJ561243, AJ561244, AJ561245, AJ561246, AJ561247, AJ561248, AJ561249, AJ561250, AJ561251, AJ561252, AJ561253, AJ561254, AJ561255, AJ561256, AJ561257, AJ561258, AJ561259, AJ561260, AJ561261, AJ561262, AJ561263, AJ561264, AJ561265, AJ561266, AJ561267, AJ561268, AJ561269, AJ561270, AJ561271, AJ561272, AJ561273, AJ561274, AJ561275, AJ561276, AJ561277, AJ561278, BX526834
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External Links
PubMed source DOI: 10.1101/gr.1555203 PMC Full Text View journal details
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