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PMID: 12835416 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Complete genome sequence of the marine planctomycete Pirellula sp. strain 1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 14 ·2003-07-08 ·Pages 8298-303

Glöckner FO, Kube M, Bauer M, Teeling H, Lombardot T, Ludwig W, Gade D, Beck A, Borzym K, Heitmann K, Rabus R, Schlesner H, Amann R, Reinhardt R

Abstract

Pirellula sp. strain 1 ("Rhodopirellula baltica") is a marine representative of the globally distributed and environmentally important bacterial order Planctomycetales. Here we report the complete genome sequence of a member of this independent phylum. With 7.145 megabases, Pirellula sp. strain 1 has the largest circular bacterial genome sequenced so far. The presence of all genes required for heterolactic acid fermentation, key genes for the interconversion of C1 compounds, and 110 sulfatases were unexpected for this aerobic heterotrophic isolate. Although Pirellula sp. strain 1 has a proteinaceous cell wall, remnants of genes for peptidoglycan synthesis were found. Genes for lipid A biosynthesis and homologues to the flagellar L- and P-ring protein indicate a former Gram-negative type of cell wall. Phylogenetic analysis of all relevant markers clearly affiliates the Planctomycetales to the domain Bacteria as a distinct phylum, but a deepest branching is not supported by our analyses.

MeSH Terms
Adaptation, Physiological/genetics Anti-Bacterial Agents/biosynthesis Bacteria/classification,genetics Bacterial Proteins/genetics Cell Compartmentation Cell Division/genetics Cell Wall/chemistry Chlamydia/genetics DNA, Bacterial/genetics DNA, Circular/genetics Environment Evolution, Molecular Fermentation/genetics Gene Library Genome, Bacterial Lipid A/biosynthesis Molecular Sequence Data Phylogeny Ribotyping Sequence Analysis, DNA
Chemicals
Anti-Bacterial Agents Bacterial Proteins DNA, Bacterial DNA, Circular Lipid A
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Glöckner F O
Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany. fog@mpi-bremen.de
Kube M
Bauer M
Teeling H
Lombardot T
Ludwig W
Gade D
Beck A
Borzym K
Heitmann K
Rabus R
Schlesner H
Amann R
Reinhardt R
References (35)
35 references, click to expand
  1. The planctomycetes: emerging models for microbial ecology, evolution and cell biology.
    Microbiology. 1995 Jul;141 ( Pt 7):1493-506 PMID: 7551018
  2. The Staden package, 1998.
    Methods Mol Biol. 2000;132:115-30 PMID: 10547834
  3. Monitoring a widespread bacterial group: in situ detection of planctomycetes with 16S rRNA-targeted probes.
    Microbiology. 1998 Dec;144 ( Pt 12):3257-66 PMID: 9884217
  4. Themes and variations in prokaryotic cell division.
    FEMS Microbiol Rev. 2000 Oct;24(4):531-48 PMID: 10978550
  5. Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell.
    Arch Microbiol. 2001 Jun;175(6):413-29 PMID: 11491082
  6. Analysis of N-acetylglucosamine metabolism in the marine bacterium Pirellula sp. strain 1 by a proteomic approach.
    Proteomics. 2002 Jun;2(6):649-55 PMID: 12112844
  7. Molecular genetic evidence for early evolutionary origin of budding peptidoglycan-less eubacteria.
    Nature. 1984 Feb 23-29;307(5953):735-7 PMID: 6700702
  8. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).
    Nature. 2002 May 9;417(6885):141-7 PMID: 12000953
  9. Three views of microbial genomes.
    Res Microbiol. 1999 Nov-Dec;150(9-10):773-7 PMID: 10673014
  10. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.
    Nature. 2000 Aug 31;406(6799):959-64 PMID: 10984043
  11. Microbial genome sequencing.
    Nature. 2000 Aug 17;406(6797):799-803 PMID: 10963611
  12. Riding the sulfur cycle--metabolism of sulfonates and sulfate esters in gram-negative bacteria.
    FEMS Microbiol Rev. 2000 Apr;24(2):135-75 PMID: 10717312
  13. Control of chromosome replication in caulobacter crescentus.
    Annu Rev Microbiol. 2002;56:625-56 PMID: 12142494
  14. Critical issues in bacterial phylogeny.
    Theor Popul Biol. 2002 Jun;61(4):423-34 PMID: 12167362
  15. Phylogeny: a non-hyperthermophilic ancestor for bacteria.
    Nature. 2002 May 16;417(6886):244 PMID: 12015592
  16. Base-calling of automated sequencer traces using phred. II. Error probabilities.
    Genome Res. 1998 Mar;8(3):186-94 PMID: 9521922
  17. Gemmata obscuriglobus, a new genus and species of the budding bacteria.
    Antonie Van Leeuwenhoek. 1984;50(3):261-8 PMID: 6486770
  18. The F0F1-type ATP synthases of bacteria: structure and function of the F0 complex.
    Annu Rev Microbiol. 1996;50:791-824 PMID: 8905099
  19. Cloning of a mucin-desulfating sulfatase gene from Prevotella strain RS2 and its expression using a Bacteroides recombinant system.
    J Bacteriol. 2000 Jun;182(11):3002-7 PMID: 10809675
  20. C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea.
    Science. 1998 Jul 3;281(5373):99-102 PMID: 9651254
  21. Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCR.
    Microbiology. 1999 Aug;145 ( Pt 8):1977-87 PMID: 10463164
  22. Improved microbial gene identification with GLIMMER.
    Nucleic Acids Res. 1999 Dec 1;27(23):4636-41 PMID: 10556321
  23. Combining diverse evidence for gene recognition in completely sequenced bacterial genomes.
    Nucleic Acids Res. 1998 Jun 15;26(12):2941-7 PMID: 9611239
  24. Isolation of Gemmata-like and Isosphaera-like planctomycete bacteria from soil and freshwater.
    Appl Environ Microbiol. 2002 Jan;68(1):417-22 PMID: 11772655
  25. Missing lithotroph identified as new planctomycete.
    Nature. 1999 Jul 29;400(6743):446-9 PMID: 10440372
  26. GenDB--an open source genome annotation system for prokaryote genomes.
    Nucleic Acids Res. 2003 Apr 15;31(8):2187-95 PMID: 12682369
  27. Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.
    Appl Environ Microbiol. 1998 Jul 1;64(7):2691-6 PMID: 9647850
  28. The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
    Nature. 1997 Nov 20;390(6657):249-56 PMID: 9384377
  29. CRITICA: coding region identification tool invoking comparative analysis.
    Mol Biol Evol. 1999 Apr;16(4):512-24 PMID: 10331277
  30. Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon.
    Appl Environ Microbiol. 1997 Jan;63(1):254-62 PMID: 8979353
  31. Gene discovery within the planctomycete division of the domain Bacteria using sequence tags from genomic DNA libraries.
    Genome Biol. 2002;3(6):RESEARCH0031 PMID: 12093378
  32. Predicted highly expressed genes of diverse prokaryotic genomes.
    J Bacteriol. 2000 Sep;182(18):5238-50 PMID: 10960111
  33. REPuter: the manifold applications of repeat analysis on a genomic scale.
    Nucleic Acids Res. 2001 Nov 15;29(22):4633-42 PMID: 11713313
  34. Functional and structural genomics using PEDANT.
    Bioinformatics. 2001 Jan;17(1):44-57 PMID: 11222261
  35. Overview of the yeast genome.
    Nature. 1997 May 29;387(6632 Suppl):7-65 PMID: 9169865
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1431443100
Email
fog@mpi-bremen.de
Published
2003-07-08
Epub
2003-00-30
Pages
8298-303
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC166223
Subset
IM
Databases
GENBANK
BX119912
External Links
PubMed source DOI: 10.1073/pnas.1431443100 PMC Full Text View journal details
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