Home LiteratureArticle Details

PMID: 11772655 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of Gemmata-like and Isosphaera-like planctomycete bacteria from soil and freshwater.

Applied and environmental microbiology ·Vol. 68 ·No. 1 ·2002-01-00 ·Pages 417-22

Wang J, Jenkins C, Webb RI, Fuerst JA

Abstract

New cultured strains of the planctomycete division (order Planctomycetales) of the domain Bacteria related to species in the genera Gemmata and Isosphaera were isolated from soil, freshwater, and a laboratory ampicillin solution. Phylogenetic analysis of the 16S rRNA gene from eight representative isolates showed that all the isolates were members of the planctomycete division. Six isolates clustered with Gemmata obscuriglobus and related strains, while two isolates clustered with Isosphaera pallida. A double-membrane-bounded nucleoid was observed in Gemmata-related isolates but not in Isosphaera-related isolates, consistent with the ultrastructures of existing species of each genus. Two isolates from this study represent the first planctomycetes successfully cultivated from soil.

MeSH Terms

Bacteria/classification,genetics,isolation & purification DNA, Ribosomal/analysis Fresh Water/microbiology Microscopy, Electron Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Soil Microbiology

Chemicals

DNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wang Jenny

Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia.

Jenkins Cheryl

Webb Richard I

Fuerst John A

References (23)

23 references, click to expand

The planctomycetes: emerging models for microbial ecology, evolution and cell biology.
Microbiology. 1995 Jul;141 ( Pt 7):1493-506 PMID: 7551018
Monitoring a widespread bacterial group: in situ detection of planctomycetes with 16S rRNA-targeted probes.
Microbiology. 1998 Dec;144 ( Pt 12):3257-66 PMID: 9884217
Novel bacterial lineages at the (sub)division level as detected by signature nucleotide-targeted recovery of 16S rRNA genes from bulk soil and rice roots of flooded rice microcosms.
Appl Environ Microbiol. 2001 Feb;67(2):623-31 PMID: 11157225
A new type of micromanipulator and microforge.
J Gen Microbiol. 1968 Dec;54(2):287-97 PMID: 4891924
Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell.
Arch Microbiol. 2001 Jun;175(6):413-29 PMID: 11491082
Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
Phylogenetic analysis of the bacterial communities in marine sediments.
Appl Environ Microbiol. 1996 Nov;62(11):4049-59 PMID: 8899989
Gemmata obscuriglobus, a new genus and species of the budding bacteria.
Antonie Van Leeuwenhoek. 1984;50(3):261-8 PMID: 6486770
Screening of a fosmid library of marine environmental genomic DNA fragments reveals four clones related to members of the order Planctomycetales.
Appl Environ Microbiol. 1998 Aug;64(8):3075-8 PMID: 9687477
Phylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA.
Microbiology. 1997 Dec;143 ( Pt 12):3913-9 PMID: 9421915
Membrane-bounded nucleoid in the eubacterium Gemmata obscuriglobus.
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8184-8 PMID: 11607213
Phylogeny of the filamentous bacterium 'Nostocoida limicola' III from activated sludge.
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):195-202 PMID: 11211260
Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method.
Appl Environ Microbiol. 1996 Oct;62(10):3787-93 PMID: 8837435
Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis.
FASEB J. 1993 Jan;7(1):232-6 PMID: 8422969
Bacterial phylogeny based on comparative sequence analysis.
Electrophoresis. 1998 Apr;19(4):554-68 PMID: 9588802
Missing lithotroph identified as new planctomycete.
Nature. 1999 Jul 29;400(6743):446-9 PMID: 10440372
Molecular microbial diversity in soils from eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation.
Appl Environ Microbiol. 1997 Jul;63(7):2647-53 PMID: 9212415
Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment.
J Bacteriol. 1992 Aug;174(15):5072-8 PMID: 1629164
Diverse uncultivated bacterial groups from soils of the arid southwestern United States that are present in many geographic regions.
Appl Environ Microbiol. 1997 Sep;63(9):3614-21 PMID: 9293013
Phylogenetic diversity, polyamine pattern and DNA base composition of members of the order Planctomycetales.
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:689-96 PMID: 10319492
Molecular evidence for genus level diversity of bacteria capable of catalyzing anaerobic ammonium oxidation.
Syst Appl Microbiol. 2000 Apr;23(1):93-106 PMID: 10879983
Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon.
Appl Environ Microbiol. 1997 Jan;63(1):254-62 PMID: 8979353
Unraveling the extent of diversity within the order Planctomycetales.
Appl Environ Microbiol. 1995 Jun;61(6):2270-5 PMID: 7793948

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.68.1.417-422.2002

Published

2002-01-00

Pages

417-22

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC126576

Subset

Databases

GENBANK

AF239692, AF239693, AF239694, AF239695, AF239696, AF239697, AF239698, AF239699

External Links

PubMed source DOI: 10.1128/AEM.68.1.417-422.2002 PMC Full Text View journal details

Analysis Services

Isolation of Gemmata-like and Isosphaera-like planctomycete bacteria from soil and freshwater.

Contact

WeChat Official Account