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PMID: 1629164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment.

Journal of bacteriology ·Vol. 174 ·No. 15 ·1992-08-00 ·Pages 5072-8

Liesack W, Stackebrandt E

Abstract

A molecular ecological study was performed on an Australian soil sample to unravel a substantial portion of the bacterial diversity. A large fragment of the 16S rRNA gene was amplified, using DNA isolated by lysing the microorganisms directly within the soil matrix, and a clone library was generated. Comparative sequence analysis of 30 clones and dot blot hybridization of 83 additional clones with defined oligonucleotide probes revealed the presence of three major groups of prokaryotes of the domain Bacteria. The first one comprises 57 clones that indicate relatives of nitrogen-fixing bacteria of the alpha-2 subclass of the class Proteobacteria; the second group of 7 clones originates from members of the order Planctomycetales that, however, reveal no close relationship to any of the described Planctomycetales species; 22 clones of the third group are indicative of members of a novel main line of descent, sharing a common ancestry with members of planctomycetes and chlamydiae.

MeSH Terms
Australia Bacteria/classification Base Sequence Cloning, Molecular DNA, Bacterial/isolation & purification Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Soil Microbiology
Chemicals
DNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liesack W
Department of Microbiology, University of Queensland, St. Lucia, Australia.
Stackebrandt E
References (19)
19 references, click to expand
  1. Separation and purification of bacteria from soil.
    Appl Environ Microbiol. 1985 Jun;49(6):1482-7 PMID: 16346816
  2. Polymerase chain reaction: applications in environmental microbiology.
    Annu Rev Microbiol. 1991;45:137-61 PMID: 1741613
  3. Genetic diversity in Sargasso Sea bacterioplankton.
    Nature. 1990 May 3;345(6270):60-3 PMID: 2330053
  4. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.
    Appl Environ Microbiol. 1988 Mar;54(3):703-711 PMID: 16347582
  5. Gemmata obscuriglobus, a new genus and species of the budding bacteria.
    Antonie Van Leeuwenhoek. 1984;50(3):261-8 PMID: 6486770
  6. 16S ribosomal DNA amplification for phylogenetic study.
    J Bacteriol. 1991 Jan;173(2):697-703 PMID: 1987160
  7. Partial 16S rRNA primary structure of five Actinomyces species: phylogenetic implications and development of an Actinomyces israelii-specific oligonucleotide probe.
    J Gen Microbiol. 1990 Jan;136(1):37-43 PMID: 1693659
  8. Bacterial evolution.
    Microbiol Rev. 1987 Jun;51(2):221-71 PMID: 2439888
  9. Membrane-bounded nucleoid in the eubacterium Gemmata obscuriglobus.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8184-8 PMID: 11607213
  10. Identification in situ and phylogeny of uncultured bacterial endosymbionts.
    Nature. 1991 May 9;351(6322):161-4 PMID: 1709451
  11. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community.
    Nature. 1990 May 3;345(6270):63-5 PMID: 1691827
  12. Eubacterial origin of chlamydiae.
    J Bacteriol. 1986 Aug;167(2):570-4 PMID: 3525517
  13. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  14. Evidence for unlinked rrn operons in the Planctomycete Pirellula marina.
    J Bacteriol. 1989 Sep;171(9):5025-30 PMID: 2768196
  15. Microbial ecology and evolution: a ribosomal RNA approach.
    Annu Rev Microbiol. 1986;40:337-65 PMID: 2430518
  16. 16S rRNA sequences of uncultivated hot spring cyanobacterial mat inhabitants retrieved as randomly primed cDNA.
    Appl Environ Microbiol. 1991 Apr;57(4):1146-51 PMID: 1711832
  17. Phylogenetic analysis of a natural marine bacterioplankton population by rRNA gene cloning and sequencing.
    Appl Environ Microbiol. 1991 Jun;57(6):1707-13 PMID: 1714704
  18. Recovery of DNA from soils and sediments.
    Appl Environ Microbiol. 1988 Dec;54(12):2908-15 PMID: 2851961
  19. Compilation of small ribosomal subunit RNA sequences.
    Nucleic Acids Res. 1990 Apr 25;18 Suppl:2237-317 PMID: 1692117
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.15.5072-5078.1992
Published
1992-08-00
Pages
5072-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206323
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1128/jb.174.15.5072-5078.1992 PMC Full Text View journal details
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