Home LiteratureArticle Details
PMID: 1264063 Published · ppublish English Journal Article

Extrachromosomal inheritance in Schizosaccharomyces pombe. I. Evidence for an extrakaryotically inherited mutation conferring resistance to antimycin.

Molecular & general genetics : MGG ·Vol. 144 ·No. 1 ·1976-02-27 ·Pages 67-73

Wolf K, Burger G, Lang B, Kaudewitz F

Abstract

In crosses of [ANTr8] with auxotrophic strains, resistance to antimycin segregates almost 50:50 in random spore analysis with a slight preponderance for the sensitivity allele. Tetrad analysis, however, shows all possible types of tetrads (2:2; 3:1; 1:3; 4:0; 0:4 resistant versus sensitive) with an excess of 2:2 segregations and sectoring of colonies on antimycin medium indicating an extrachromosomal mode of inheritance. The overall ratio of resistant versus sensitive spores is the same as compared with random spore data. Using a mutant blocked in meiosis (mei 1) mitotic segregation of stable diploids is achieved, leading to a ratio of 20% resistant to 80% sensitive clones. Possible reasons for the bias in transmission of the resistance determinant is discussed.

MeSH Terms
Antimycin A/pharmacology Ascomycota/metabolism Crosses, Genetic DNA, Mitochondrial/metabolism Diploidy Drug Resistance, Microbial Extrachromosomal Inheritance Haploidy Mutation Recombination, Genetic Schizosaccharomyces/drug effects,metabolism Spores, Fungal
Chemicals
DNA, Mitochondrial Antimycin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wolf K
Burger G
Lang B
Kaudewitz F
References (24)
24 references, click to expand
  1. INDUCTION OF PETITE MUTATION AND INHIBITION OF SYNTHESIS OF RESPIRATORY ENZYMES IN VARIOUS YEASTS.
    Antonie Van Leeuwenhoek. 1964;30:1-9 PMID: 14160070
  2. Studies on energy-linked reactions: isolation, characterisation and genetic analysis of trialkyl-tin-resistant mutants of Saccharomyces cerevisiae.
    Eur J Biochem. 1975 Feb 21;51(2):377-92 PMID: 125200
  3. Genes involved in mating type expression of fission yeast.
    Mol Gen Genet. 1973 May 28;122(4):339-43 PMID: 4716873
  4. Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.
    J Bacteriol. 1972 Jan;109(1):196-202 PMID: 4550660
  5. Effect of carbon source on the replication and transmission of yeast mitochondrial genomes.
    Mol Gen Genet. 1974;133(2):87-104 PMID: 4614066
  6. Genetics of a primaquin-resistant yeast.
    J Gen Microbiol. 1975 Jul;89(1):1-10 PMID: 1097590
  7. Mitochondrial DNA in the fission yeast Schizosaccharomyces pombe.
    Biochim Biophys Acta. 1969 Dec 16;195(2):579-81 PMID: 5366939
  8. Mitochondrial genetics IX: A model for recombination and segregation of mitochondrial genomes in saccharomyces cerevisiae.
    Genetics. 1974 Sep;78(1):415-37 PMID: 4613610
  9. Non-Mendelian mutation allowing ureidosuccinic acid uptake in yeast.
    J Bacteriol. 1971 May;106(2):519-22 PMID: 5573734
  10. [Study of mitotic conversions at level of ad-9 gene in Schizosaccharomyces pombe].
    Mutat Res. 1970 Jan;9(1):41-58 PMID: 5410809
  11. Characterization of ICR-170-induced mutations in Schizosaccharomyces pombe.
    Mutat Res. 1970 Feb;9(2):199-212 PMID: 5413678
  12. Mitochondrial genetics, circular DNA and the mechanism of the petite mutation in yeast.
    Genet Res. 1974 Aug;24(1):43-57 PMID: 4611825
  13. LETHALITY OF THE PETITE MUTATION IN PETITE NEGATIVE YEASTS.
    Antonie Van Leeuwenhoek. 1964;30:442-54 PMID: 14274138
  14. Yeast episome: oligomycin resistance associated with a small covalently closed non-mitochondrial circular DNA.
    Biochem Biophys Res Commun. 1974 Nov 27;61(2):462-9 PMID: 4616687
  15. Respiratory metabolism of a "petite negative"yeast Schizosaccharomyces pombe 972h-.
    J Bacteriol. 1970 Oct;104(1):473-81 PMID: 4394400
  16. Effects of glucose repression of the transmission and recombination of mitochondrial genes in yeast (Saccharomyces cerevisiae).
    Genetics. 1975 Aug;80(4):695-709 PMID: 1104405
  17. Influence of ethidium bromide on respiration in Schizosaccharomyces pombe.
    Mol Gen Genet. 1971;110(4):361-6 PMID: 5574428
  18. Respiration deficient mutants of Schizosaccharomyces Pombe I.
    Mol Gen Genet. 1971;110(2):101-9 PMID: 4323756
  19. Studies on the mechanism of electron trasport in the bc1-segment of the respiratory chain in yeast. III. Isolation and characterization of an antimycin resistant mutant ANT 8 in Schizosaccharomyces pombe.
    Mol Gen Genet. 1975;137(4):353-63 PMID: 1186659
  20. The isolation of an antimycin-resistant mutant of orulopsis utilis.
    J Biol Chem. 1968 May 25;243(10):2545-9 PMID: 4297262
  21. The mitochondrial genome of wild-type yeast cells. IV. Genes and spacers.
    J Mol Biol. 1974 Jul 15;86(4):825-41 PMID: 4610147
  22. Heterogeneity of mitochondrial DNA from Saccharomyces carlsbergensis. Denaturation mapping by electron microscopy.
    Nucleic Acids Res. 1975 Feb;2(2):197-210 PMID: 1121424
  23. Mitochondrial nucleic acids.
    Annu Rev Biochem. 1972;41:333-76 PMID: 4563438
  24. Antimycin-insensitive mutants of Candida utilis. I. Isolation and characterization of mutant 28.
    Biochim Biophys Acta. 1973 Apr 27;305(1):67-79 PMID: 4146344
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1976-02-27
Pages
67-73
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2