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PMID: 12615939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A plant-specific dynamin-related protein forms a ring at the chloroplast division site.

The Plant cell ·Vol. 15 ·No. 3 ·2003-03-00 ·Pages 655-65

Miyagishima SY, Nishida K, Mori T, Matsuzaki M, Higashiyama T, Kuroiwa H, Kuroiwa T

Abstract

Chloroplasts have retained the bacterial FtsZ for division, whereas mitochondria lack FtsZ except in some lower eukaryotes. Instead, mitochondrial division involves a dynamin-related protein, suggesting that chloroplasts retained the bacterial division system, whereas a dynamin-based system replaced the bacterial system in mitochondria during evolution. In this study, we identified a novel plant-specific group of dynamins from the primitive red alga Cyanidioschyzon merolae. Synchronization of chloroplast division and immunoblot analyses showed that the protein (CmDnm2) associates with the chloroplast only during division. Immunocytochemical analyses showed that CmDnm2 appears in cytoplasmic patches just before chloroplast division and is recruited to the cytosolic side of the chloroplast division site to form a ring in the late stage of division. The ring constricts until division is complete, after which it disappears. These results show that a dynamin-related protein also participates in chloroplast division and that its behavior differs from that of FtsZ and plastid-dividing rings that form before constriction at the site of division. Combined with the results of a recent study of mitochondrial division in Cyanidioschyzon, our findings led us to hypothesize that when first established in lower eukaryotes, mitochondria and chloroplasts divided using a very similar system that included the FtsZ ring, the plastid-dividing/mitochondrion-dividing ring, and the dynamin ring.

MeSH Terms
Algal Proteins/genetics,metabolism Amino Acid Sequence Arabidopsis Proteins/genetics,metabolism Cell Division/genetics,physiology Chloroplasts/metabolism,ultrastructure Cytosol/metabolism Dynamin II/genetics,metabolism Dynamins/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Immunohistochemistry Microscopy, Immunoelectron Molecular Sequence Data Phylogeny Plant Proteins/genetics,metabolism Rhodophyta/genetics,metabolism,ultrastructure Sequence Homology, Amino Acid
Chemicals
Algal Proteins Arabidopsis Proteins FTSZ protein, Arabidopsis Plant Proteins GTP-Binding Proteins Dynamin II Dynamins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Miyagishima Shin-ya
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan. miyagi@biol.s.u-tokyo.ac.jp
Nishida Keiji
Mori Toshiyuki
Matsuzaki Motomichi
Higashiyama Tetsuya
Kuroiwa Haruko
Kuroiwa Tsuneyoshi
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.009373
Email
miyagi@biol.s.u-tokyo.ac.jp
Published
2003-03-00
Pages
655-65
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150020
Subset
IM
Databases
GENBANK
AB094119
External Links
PubMed source DOI: 10.1105/tpc.009373 PMC Full Text View journal details
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