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PMID: 11179417 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Division of mitochondria requires a novel DNM1-interacting protein, Net2p.

Molecular biology of the cell ·Vol. 12 ·No. 2 ·2001-00-00 ·Pages 309-21

Cerveny KL, McCaffery JM, Jensen RE

Abstract

Mitochondria are dynamic organelles that undergo frequent division and fusion, but the molecular mechanisms of these two events are not well understood. Dnm1p, a mitochondria-associated, dynamin-related GTPase was previously shown to mediate mitochondrial fission. Recently, a genome-wide yeast two-hybrid screen identified an uncharacterized protein that interacts with Dnm1p. Cells disrupted in this new gene, which we call NET2, contain a single mitochondrion that consists of a network formed by interconnected tubules, similar to the phenotype of dnm1 Delta cells. NET2 encodes a mitochondria-associated protein with a predicted coiled-coil region and six WD-40 repeats. Immunofluorescence microscopy indicates that Net2p is located in distinct, dot-like structures along the mitochondrial surface, many of which colocalize with the Dnm1 protein. Fluorescence and immunoelectron microscopy shows that Dnm1p and Net2p preferentially colocalize at constriction sites along mitochondrial tubules. Our results suggest that Net2p is a new component of the mitochondrial division machinery.

MeSH Terms
Actins/metabolism,ultrastructure Adaptor Proteins, Signal Transducing Base Sequence Carrier Proteins/genetics,metabolism Cytoskeleton/genetics,ultrastructure Fungal Proteins/genetics,metabolism GTP Phosphohydrolases/genetics,metabolism Membrane Proteins/genetics,metabolism Mitochondria/genetics,metabolism,ultrastructure Mitochondrial Proteins Molecular Sequence Data Mutation Open Reading Frames Repetitive Sequences, Amino Acid Saccharomyces cerevisiae Proteins Yeasts/genetics,metabolism
Chemicals
Actins Adaptor Proteins, Signal Transducing Carrier Proteins Fungal Proteins MDV1 protein, S cerevisiae Membrane Proteins Mitochondrial Proteins Saccharomyces cerevisiae Proteins FZO1 protein, S cerevisiae GTP Phosphohydrolases DNM1 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cerveny K L
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
McCaffery J M
Jensen R E
References (47)
47 references, click to expand
  1. Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules.
    Mol Cell Biol. 1998 Jan;18(1):178-87 PMID: 9418865
  2. Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA.
    Mol Biol Cell. 1997 Jul;8(7):1233-42 PMID: 9243504
  3. Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p.
    J Cell Biol. 1998 Jun 15;141(6):1371-81 PMID: 9628893
  4. Mitochondria and apoptosis.
    Science. 1998 Aug 28;281(5381):1309-12 PMID: 9721092
  5. The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast.
    J Cell Biol. 1998 Oct 19;143(2):333-49 PMID: 9786946
  6. Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p.
    J Cell Biol. 1998 Oct 19;143(2):359-73 PMID: 9786948
  7. Mitochondrial diseases in man and mouse.
    Science. 1999 Mar 5;283(5407):1482-8 PMID: 10066162
  8. Impairment of dynamin's GAP domain stimulates receptor-mediated endocytosis.
    Nature. 1999 Apr 8;398(6727):481-6 PMID: 10206643
  9. Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid.
    Nature. 1999 Sep 9;401(6749):133-41 PMID: 10490020
  10. Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape.
    J Cell Biol. 1999 Nov 15;147(4):699-706 PMID: 10562274
  11. The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast.
    Nat Cell Biol. 1999 Sep;1(5):298-304 PMID: 10559943
  12. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.
    Nature. 2000 Feb 10;403(6770):623-7 PMID: 10688190
  13. A functional link between dynamin and the actin cytoskeleton at podosomes.
    J Cell Biol. 2000 Jul 24;150(2):377-89 PMID: 10908579
  14. Gag3p, an outer membrane protein required for fission of mitochondrial tubules.
    J Cell Biol. 2000 Oct 16;151(2):333-40 PMID: 11038180
  15. Mdv1p is a WD repeat protein that interacts with the dynamin-related GTPase, Dnm1p, to trigger mitochondrial division.
    J Cell Biol. 2000 Oct 16;151(2):353-66 PMID: 11038182
  16. Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral membrane component Fis1p.
    J Cell Biol. 2000 Oct 16;151(2):367-80 PMID: 11038183
  17. Yeast mitochondrial dynamics: fusion, division, segregation, and shape.
    Microsc Res Tech. 2000 Dec 15;51(6):573-83 PMID: 11169859
  18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  19. Mitochondrion of yeast: ultrastructural evidence for one giant, branched organelle per cell.
    Science. 1973 Aug 24;181(4101):749-51 PMID: 4579683
  20. Internal sequence repeats and the path of polypeptide in mitochondrial ADP/ATP translocase.
    FEBS Lett. 1982 Aug 2;144(2):250-4 PMID: 6288471
  21. Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.
    J Biol Chem. 1982 Nov 10;257(21):13028-33 PMID: 6290489
  22. Generation of protein-reactive antibodies by short peptides is an event of high frequency: implications for the structural basis of immune recognition.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4949-53 PMID: 6192445
  23. Immunochemical identification of membrane proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    Methods Enzymol. 1983;96:192-205 PMID: 6228704
  24. Fluorescence microscopic studies of mitochondrial nucleoids during meiosis and sporulation in the yeast, Saccharomyces cerevisiae.
    J Cell Sci. 1984 Mar;66:21-38 PMID: 6378943
  25. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
    Gene. 1987;57(2-3):267-72 PMID: 3319781
  26. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
    Mol Cell Biol. 1988 May;8(5):2159-65 PMID: 2455217
  27. Biogenesis of mitochondria.
    Annu Rev Cell Biol. 1988;4:289-333 PMID: 2461720
  28. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  29. The major 45-kDa protein of the yeast mitochondrial outer membrane is not essential for cell growth or mitochondrial function.
    J Biol Chem. 1989 Dec 15;264(35):21091-6 PMID: 2687271
  30. Behavior of mitochondria in the living cell.
    Int Rev Cytol. 1990;122:1-63 PMID: 2246114
  31. Staining of actin with fluorochrome-conjugated phalloidin.
    Methods Enzymol. 1991;194:729-31 PMID: 2005819
  32. Predicting coiled coils from protein sequences.
    Science. 1991 May 24;252(5009):1162-4 PMID: 2031185
  33. Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system.
    FASEB J. 1993 Jul;7(10):957-63 PMID: 8344494
  34. MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway.
    J Cell Biol. 1993 Sep;122(5):1003-12 PMID: 8354690
  35. Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.
    Mol Biol Cell. 1993 Dec;4(12):1277-94 PMID: 8167410
  36. Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria.
    Microsc Res Tech. 1994 Feb 15;27(3):198-219 PMID: 8204911
  37. Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity.
    Mol Biol Cell. 1994 Jul;5(7):807-18 PMID: 7812049
  38. Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding.
    Nature. 1995 Mar 9;374(6518):190-2 PMID: 7877694
  39. DNM1, a dynamin-related gene, participates in endosomal trafficking in yeast.
    J Cell Biol. 1995 Aug;130(3):553-66 PMID: 7622557
  40. Organelle-cytoskeletal interactions: actin mutations inhibit meiosis-dependent mitochondrial rearrangement in the budding yeast Saccharomyces cerevisiae.
    Mol Biol Cell. 1995 Oct;6(10):1381-96 PMID: 8573793
  41. The function of dynamin in endocytosis.
    Curr Opin Neurobiol. 1995 Oct;5(5):559-65 PMID: 8580706
  42. Prediction and analysis of coiled-coil structures.
    Methods Enzymol. 1996;266:513-25 PMID: 8743703
  43. Gene identification using the yeast two-hybrid system.
    Methods Enzymol. 1996;273:331-47 PMID: 8791622
  44. Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant.
    Mol Biol Cell. 1996 Jun;7(6):985-99 PMID: 8817003
  45. Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit.
    Biochemistry. 1996 Nov 5;35(44):13985-94 PMID: 8909296
  46. Developmentally regulated mitochondrial fusion mediated by a conserved, novel, predicted GTPase.
    Cell. 1997 Jul 11;90(1):121-9 PMID: 9230308
  47. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
    Yeast. 1998 Jan 30;14(2):115-32 PMID: 9483801
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.12.2.309
Published
2001-00-00
Pages
309-21
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC30945
Subset
IM
Grants
NIGMS NIH HHS · R01 GM054021 · United States
NIGMS NIH HHS · T32 GM007445 · United States
NIGMS NIH HHS · 2T32GM07445 · United States
NIGMS NIH HHS · R01-GM54021 · United States
External Links
PubMed source DOI: 10.1091/mbc.12.2.309 PMC Full Text View journal details
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