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PMID: 9628893 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p.

The Journal of cell biology ·Vol. 141 ·No. 6 ·1998-06-15 ·Pages 1371-81

Boldogh I, Vojtov N, Karmon S, Pon LA

Abstract

Transfer of mitochondria to daughter cells during yeast cell division is essential for viable progeny. The actin cytoskeleton is required for this process, potentially as a track to direct mitochondrial movement into the bud. Sedimentation assays reveal two different components required for mitochondria-actin interactions: (1) mitochondrial actin binding protein(s) (mABP), a peripheral mitochondrial outer membrane protein(s) with ATP-sensitive actin binding activity, and (2) a salt-inextractable, presumably integral, membrane protein(s) required for docking of mABP on the organelle. mABP activity is abolished by treatment of mitochondria with high salt. Addition of either the salt-extracted mitochondrial peripheral membrane proteins (SE), or a protein fraction with ATP-sensitive actin-binding activity isolated from SE, to salt-washed mitochondria restores this activity. mABP docking activity is saturable, resistant to high salt, and inhibited by pre-treatment of salt-washed mitochondria with papain. Two integral mitochondrial outer membrane proteins, Mmm1p (Burgess, S.M., M. Delannoy, and R.E. Jensen. 1994. J.Cell Biol. 126:1375-1391) and Mdm10p, (Sogo, L.F., and M.P. Yaffe. 1994. J.Cell Biol. 126:1361- 1373) are required for these actin-mitochondria interactions. Mitochondria isolated from an mmm1-1 temperature-sensitive mutant or from an mdm10 deletion mutant show no mABP activity and no mABP docking activity. Consistent with this, mitochondrial motility in vivo in mmm1-1 and mdm10Delta mutants appears to be actin independent. Depolymerization of F-actin using latrunculin-A results in loss of long-distance, linear movement and a fivefold decrease in the velocity of mitochondrial movement. Mitochondrial motility in mmm1-1 and mdm10Delta mutants is indistinguishable from that in latrunculin-A-treated wild-type cells. We propose that Mmm1p and Mdm10p are required for docking of mABP on the surface of yeast mitochondria and coupling the organelle to the actin cytoskeleton.

MeSH Terms
Actins/metabolism Bridged Bicyclo Compounds, Heterocyclic/pharmacology Cytoskeleton/metabolism Fungal Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Mitochondria/drug effects,metabolism,physiology Mutagenesis Potassium Chloride Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Thiazoles/pharmacology Thiazolidines
Chemicals
Actins Bridged Bicyclo Compounds, Heterocyclic Fungal Proteins MDM10 protein, S cerevisiae MMM1 protein, S cerevisiae Membrane Proteins Saccharomyces cerevisiae Proteins Thiazoles Thiazolidines Potassium Chloride latrunculin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boldogh I
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Vojtov N
Karmon S
Pon L A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.141.6.1371
Published
1998-06-15
Pages
1371-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132797
Subset
IM
Grants
NIGMS NIH HHS · GM45735 · United States
External Links
PubMed source DOI: 10.1083/jcb.141.6.1371 PMC Full Text View journal details
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