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PMID: 9024686 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mdm12p, a component required for mitochondrial inheritance that is conserved between budding and fission yeast.

The Journal of cell biology ·Vol. 136 ·No. 3 ·1997-02-10 ·Pages 545-53

Berger KH, Sogo LF, Yaffe MP

Abstract

Saccharomyces cerevisiae cells lacking the MDM12 gene product display temperature-sensitive growth and possess abnormally large, round mitochondria that are defective for inheritance by daughter buds. Analysis of the wild-type MDM12 gene revealed its product to be a 31-kD polypeptide that is homologous to a protein of the fission yeast Schizosaccharomyces pombe. When expressed in S. cerevisiae, the S. pombe Mdm12p homolog conferred a dominant-negative phenotype of giant mitochondria and aberrant mitochondrial distribution, suggesting partial functional conservation of Mdm12p activity between budding and fission yeast. The S. cerevisiae Mdm12p was localized by indirect immunofluorescence microscopy and by subcellular fractionation and immunodetection to the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species.

MeSH Terms
Base Sequence Cell Membrane/metabolism DNA, Fungal Fungal Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Mitochondria/metabolism,ultrastructure Molecular Sequence Data Phenotype Repressor Proteins Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces/metabolism Sequence Homology, Amino Acid Spores, Fungal
Chemicals
DNA, Fungal Fungal Proteins MDM10 protein, S cerevisiae Membrane Proteins Repressor Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berger K H
Department of Biology, University of California, San Diego, La Jolla 92093-0347, USA.
Sogo L F
Yaffe M P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.136.3.545
Published
1997-02-10
Pages
545-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2134291
Subset
IM
Grants
NIGMS NIH HHS · GM16173 · United States
NIGMS NIH HHS · GM44614 · United States
Databases
GENBANK
U62252
External Links
PubMed source DOI: 10.1083/jcb.136.3.545 PMC Full Text View journal details
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