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PMID: 12609863 Published · ppublish English Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Validation Study

Force generation by actin polymerization II: the elastic ratchet and tethered filaments.

Biophysical journal ·Vol. 84 ·No. 3 ·2003-03-00 ·Pages 1591-605

Mogilner A, Oster G

Abstract

The motion of many intracellular pathogens is driven by the polymerization of actin filaments. The propulsive force developed by the polymerization process is thought to arise from the thermal motions of the polymerizing filament tips. Recent experiments suggest that the nucleation of actin filaments involves a phase when the filaments are attached to the pathogen surface by a protein complex. Here we extend the "elastic ratchet model" of Mogilner and Oster to incorporate these new findings. We apply this "tethered ratchet" model to derive the force-velocity relation for Listeria and discuss relations of our theoretical predictions to experimental measurements. We also discuss "symmetry breaking" dynamics observed in ActA-coated bead experiments, and the implications of the model for lamellipodial protrusion in migrating cells.

MeSH Terms
Actins/chemistry Bacterial Proteins/chemistry,physiology Coated Materials, Biocompatible Computer Simulation Elasticity Listeria/chemistry,physiology Macromolecular Substances Microspheres Models, Biological Models, Molecular Molecular Motor Proteins/chemistry,physiology Motion Polymers/chemistry Protein Conformation Stochastic Processes Stress, Mechanical
Chemicals
Actins Bacterial Proteins Coated Materials, Biocompatible Macromolecular Substances Molecular Motor Proteins Polymers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mogilner Alex
Department of Mathematics and Center for Genetics and Development, University of California, Davis 95616, USA.
Oster George
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(03)74969-8
Published
2003-03-00
Pages
1591-605
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1302730
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059875 · United States
NIGMS NIH HHS · U54 GM064346 · United States
NIGMS NIH HHS · GM59875-02 · United States
NIGMS NIH HHS · U54 GM64346 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(03)74969-8 PMC Full Text View journal details
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