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PMID: 10051592 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protrusive growth from giant liposomes driven by actin polymerization.

Miyata H, Nishiyama S, Akashi K, Kinosita K

Abstract

Development of protrusions in the cell is indispensable in the process of cell motility. Membrane protrusion has long been suggested to occur as a result of actin polymerization immediately beneath the cell membrane at the leading edge, but elucidation of the mechanism is insufficient because of the complexity of the cell. To study the mechanism, we prepared giant liposomes containing monomeric actin (100 or 200 microM) and introduced KCl into individual liposomes by an electroporation technique. On the electroporation, the giant liposomes deformed. Most importantly, protrusive structure grew from the liposomes containing 200 microM actin at rates (ranging from 0.3 to 0.7 micrometer/s) similar to those obtained in the cell. The deformation occurred in a time range (30 approximately 100 s) similar to that of actin polymerization monitored in a cuvette (ca. 50 s). Concomitant with deformation, Brownian motion of micron-sized particles entrapped in the liposomes almost ceased. From these observations, we conclude that actin polymerization in the liposomes caused the protrusive formation.

MeSH Terms
Actins/chemistry,isolation & purification,metabolism Animals Electroporation Liposomes/chemistry Macromolecular Substances Microscopy, Phase-Contrast Muscle, Skeletal Potassium Chloride Rabbits
Chemicals
Actins Liposomes Macromolecular Substances Potassium Chloride
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miyata H
Department of Physics, Faculty of Science and Technology, Keio University. 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. miyata@bio.phys.tohoku.ac.jp
Nishiyama S
Akashi K
Kinosita K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1999-03-02
Pages
2048-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26734
Subset
IM
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