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PMID: 12586900 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants.

Plant physiology ·Vol. 131 ·No. 2 ·2003-02-00 ·Pages 763-72

Podkowinski J, Jelenska J, Sirikhachornkit A, Zuther E, Haselkorn R, Gornicki P

Abstract

Expression of cytosolic and plastid acetyl-coenzyme A carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5' splice site for the first intron accumulates to its highest level in roots. Cytosolic ACCase mRNA also consists of two species, one of which is present at approximately a constant level, whereas the other accumulates to a high level in the lower sheath section. It is likely that different promoters are also responsible for the two forms of cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase mRNAs in the sheath section of the plant are similar. ACCase protein level is significantly lower in the leaf blades, in parallel with changes in the total ACCase mRNA level. Homoeologous ACCase genes show the same expression patterns and similar mRNA levels, suggesting that none of the genes was silenced or acquired new tissue specificity after polyploidization.

MeSH Terms
Acetyl-CoA Carboxylase/genetics,metabolism Blotting, Northern Cytosol/enzymology Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Multigene Family Phosphoglycerate Kinase/metabolism Plant Leaves/enzymology,genetics Plastids/enzymology Polyploidy RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Triticum/enzymology,genetics,growth & development
Chemicals
RNA, Messenger Phosphoglycerate Kinase Acetyl-CoA Carboxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Podkowinski Jan
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637.
Jelenska Joanna
Sirikhachornkit Anchalee
Zuther Ellen
Haselkorn Robert
Gornicki Piotr
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.013169
Published
2003-02-00
Pages
763-72
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC166852
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.013169 PMC Full Text View journal details
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