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PMID: 12554882 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure and function analysis of the poliovirus cis-acting replication element (CRE).

RNA (New York, N.Y.) ·Vol. 9 ·No. 1 ·2003-01-00 ·Pages 124-37

Goodfellow IG, Kerrigan D, Evans DJ

Abstract

The poliovirus cis-acting replication element (CRE) templates the uridylylation of VPg, the protein primer for genome replication. The CRE is a highly conserved structural RNA element in the enteroviruses and located within the polyprotein-coding region of the genome. We have determined the native structure of the CRE, defined the regions of the structure critical for activity, and investigated the influence of genomic location on function. Our results demonstrate that a 14-nucleotide unpaired terminal loop, presented on a suitably stable stem, is all that is required for function. These conclusions complement the recent analysis of the 14-nucleotide terminal loop in the CRE of human rhinovirus type 14. The CRE can be translocated to the 5' noncoding region of the genome, at least 3.7-kb distant from the native location, without adversely influencing activity, and CRE duplications do not adversely influence replication. We do not have evidence for a specific interaction between the CRE and the RNA-binding 3CD(pro) complex, an essential component of the uridylylation reaction, and the mechanism by which the CRE is coordinated and orientated during the reaction remains unclear. These studies provide a detailed overview of the structural determinants required for CRE function, and will facilitate a better understanding of the requirements for picornavirus replication.

MeSH Terms
Base Sequence Electrophoretic Mobility Shift Assay HeLa Cells Humans Molecular Sequence Data Mutagenesis Nucleic Acid Conformation Poliovirus/genetics,physiology RNA, Viral/chemistry,genetics Structure-Activity Relationship
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goodfellow Ian G
Faculty of Biomedical and Life Sciences, Division of Virology, University of Glasgow, Church Street, UK.
Kerrigan David
Evans David J
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.2950603
Published
2003-01-00
Pages
124-37
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370376
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.2950603 PMC Full Text View journal details
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