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PMID: 11463383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Poliovirus RNA replication requires genome circularization through a protein-protein bridge.

Molecular cell ·Vol. 7 ·No. 3 ·2001-03-00 ·Pages 581-91

Herold J, Andino R

Abstract

The mechanisms and factors involved in the replication of positive stranded RNA viruses are still unclear. Using poliovirus as a model, we show that a long-range interaction between ribonucleoprotein (RNP) complexes formed at the ends of the viral genome is necessary for RNA replication. Initiation of negative strand RNA synthesis requires a 3' poly(A) tail. Strikingly, it also requires a cloverleaf-like RNA structure located at the other end of the genome. An RNP complex formed around the 5' cloverleaf RNA structure interacts with the poly(A) binding protein bound to the 3' poly(A) tail, thus linking the ends of the viral RNA and effectively circularizing it. Formation of this circular RNP complex is required for initiation of negative strand RNA synthesis. RNA circularization may be a general replication mechanism for positive stranded RNA viruses.

MeSH Terms
Base Sequence Cell Line Cross-Linking Reagents DNA, Circular/chemistry,genetics,metabolism DNA-Binding Proteins Genome, Viral Heterogeneous-Nuclear Ribonucleoproteins Humans Mutation/genetics Nucleic Acid Conformation Poliovirus/genetics Poly A/genetics,metabolism Poly(A)-Binding Proteins Protein Binding RNA, Messenger/chemistry,genetics,metabolism RNA, Viral/biosynthesis,genetics,metabolism RNA-Binding Proteins/genetics,metabolism Regulatory Sequences, Nucleic Acid/genetics Ribonucleoproteins/genetics,metabolism Virus Replication/genetics,physiology
Chemicals
Cross-Linking Reagents DNA, Circular DNA-Binding Proteins Heterogeneous-Nuclear Ribonucleoproteins PCBP1 protein, human Poly(A)-Binding Proteins RNA, Messenger RNA, Viral RNA-Binding Proteins Ribonucleoproteins Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Herold J
Department of Microbiology and Immunology, University of California, San Francisco, 94143, USA.
Andino R
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-2765
DOI
10.1016/s1097-2765(01)00205-2
Published
2001-03-00
Pages
581-91
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC7129834
Subset
IM
Grants
NIAID NIH HHS · N01AI40085 · United States
NIAID NIH HHS · AI 40085 · United States
External Links
PubMed source DOI: 10.1016/s1097-2765(01)00205-2 PMC Full Text View journal details
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