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PMID: 12533491 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosomal arm replacement in Streptomyces griseus.

Journal of bacteriology ·Vol. 185 ·No. 3 ·2003-02-00 ·Pages 1120-4

Uchida T, Miyawaki M, Kinashi H

Abstract

UV irradiation of Streptomyces griseus 2247 yielded a new chromosomal deletion mutant, MM9. Restriction and sequencing analysis revealed that homologous recombination between two similar lipoprotein-like open reading frames, which are located 450 and 250 kb from the left and right ends, respectively, caused chromosomal arm replacement. As a result, new 450-kb terminal inverted repeats (TIRs) were formed in place of the original 24-kb TIRs. Frequent homologous recombinations in Streptomyces strains suggest that telomere deletions can usually be repaired by recombinational DNA repair functioning between the intact and deleted TIR sequences on the same chromosome.

MeSH Terms
Base Sequence Chromosome Deletion Chromosome Inversion Chromosomes, Bacterial/genetics DNA Repair Molecular Sequence Data Open Reading Frames Streptomyces griseus/genetics Terminal Repeat Sequences
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Uchida Tetsuya
Department of Molecular Biotechnology, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
Miyawaki Mariko
Kinashi Haruyasu
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.3.1120-1124.2003
Published
2003-02-00
Pages
1120-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC142817
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.3.1120-1124.2003 PMC Full Text View journal details
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