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PMID: 11889104 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical map of the linear chromosome of Streptomyces hygroscopicus 10-22 deduced by analysis of overlapping large chromosomal deletions.

Journal of bacteriology ·Vol. 184 ·No. 7 ·2002-04-00 ·Pages 1958-65

Pang X, Zhou X, Sun Y, Deng Z

Abstract

The chromosomal DNA of Streptomyces hygroscopicus 10-22, a derivative of strain 5102-6, was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestions with AseI gave 11 fragments with a total length of ca. 7.36 Mb. The AseI sites were mapped by analysis of overlapping chromosomal deletions in different mutants and confirmed by Southern hybridizations using partially digested genome fragments and linking cosmids as probes. PFGE analysis of DNA with and without proteinase K treatment, together with the hybridization results, suggested a linear organization with terminal proteins and large terminal inverted repeats. Some deletion mutants had circular chromosomes.

MeSH Terms
Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial Cosmids/genetics Deoxyribonucleases, Type II Site-Specific/metabolism Plasmids/genetics Restriction Mapping Streptomyces/genetics
Chemicals
endodeoxyribonuclease VspI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pang Xiuhua
Bio-X Life Science Research Center, Shanghai Jiaotong University, Shanghai 200030, China.
Zhou Xiufen
Sun Yuhui
Deng Zixin
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.7.1958-1965.2002
Published
2002-04-00
Pages
1958-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC134931
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.184.7.1958-1965.2002 PMC Full Text View journal details
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