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PMID: 12511500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

FlhD/FlhC is a regulator of anaerobic respiration and the Entner-Doudoroff pathway through induction of the methyl-accepting chemotaxis protein Aer.

Journal of bacteriology ·Vol. 185 ·No. 2 ·2003-01-00 ·Pages 534-43

Prüss BM, Campbell JW, Van Dyk TK, Zhu C, Kogan Y, Matsumura P

Abstract

The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon gene regulation were investigated. Altogether, the flhD::Kan insertion altered the expression of 29 operons of known function. Among them was Aer, which in turn regulated a subset of these operons, namely, the ones involved in anaerobic respiration and the Entner-Doudoroff pathway. In addition, FlhD/FlhC repressed enzymes involved in aerobic respiration and regulated many other metabolic enzymes and transporters in an Aer-independent manner. Expression of 12 genes of uncharacterized function was also affected. FlhD increased gltBD, gcvTHP, and ompT expression. The regulation of half of these genes was subsequently confirmed with reporter gene fusions, enzyme assays, and real-time PCR. Growth phenotypes of flhD and flhC mutants were determined with Phenotype MicroArrays and correlated with gene expression.

MeSH Terms
Anaerobiosis Bacterial Proteins Carrier Proteins/genetics,metabolism Chemotaxis DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics,metabolism,physiology Escherichia coli Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Intercellular Signaling Peptides and Proteins Membrane Proteins/genetics,metabolism Methyl-Accepting Chemotaxis Proteins Oligonucleotide Array Sequence Analysis/instrumentation,methods Operon Oxygen Consumption Pentose Phosphate Pathway Phosphogluconate Dehydrogenase/genetics,metabolism Trans-Activators/genetics,metabolism
Chemicals
Aer protein, E coli Bacterial Proteins Carrier Proteins DNA-Binding Proteins Escherichia coli Proteins Intercellular Signaling Peptides and Proteins Membrane Proteins Methyl-Accepting Chemotaxis Proteins Trans-Activators flhC protein, E coli flhD protein, E coli Phosphogluconate Dehydrogenase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Prüss Birgit M
Department of Microbiology and Immunology, University of Illinois at Chicago, 60612-7344, USA. pruess@UIC.EDU
Campbell John W
Van Dyk Tina K
Zhu Charles
Kogan Yakov
Matsumura Philip
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.2.534-543.2003
Email
pruess@uic.edu
Published
2003-01-00
Pages
534-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC145316
Subset
IM
Grants
NIGMS NIH HHS · GM59484 · United States
External Links
PubMed source DOI: 10.1128/JB.185.2.534-543.2003 PMC Full Text View journal details
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