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PMID: 12490715 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The centrosomal kinase Aurora-A/STK15 interacts with a putative tumor suppressor NM23-H1.

Nucleic acids research ·Vol. 30 ·No. 24 ·2002-12-15 ·Pages 5465-75

Du J, Hannon GJ

Abstract

Alterations in the activity of the centrosomal kinase, Aurora-A/STK15, have been implicated in centrosome amplification, genome instability and cellular transformation. How STK15 participates in all of these processes remains largely mysterious. The activity of STK15 is regulated by phosphorylation and ubiquitin-mediated degradation, and physically interacts with protein phosphatase 1 (PP1) and CDC20. However, the precise roles of these modifications and interactions have yet to be fully appreciated. Here we show that STK15 associates with a putative tumor and metastasis suppressor, NM23-H1. STK15 and NM23 were initially found to interact in yeast in a two-hybrid assay. Association of these proteins in human cells was confirmed by co-immunoprecipitation from cell lysates and biochemical fractionation indicating that STK15 and NM23-H1 are present in a stable, physical complex. Notably, SKT15 and NM23 both localize to centrosomes throughout the cell cycle irrespective of the integrity of the microtubule network in normal human fibroblasts.

MeSH Terms
Aurora Kinase A Aurora Kinases Cell Line Centrosome/enzymology,metabolism HeLa Cells Humans Immunoblotting Microscopy, Fluorescence Monomeric GTP-Binding Proteins/genetics,metabolism NM23 Nucleoside Diphosphate Kinases Nucleoside-Diphosphate Kinase Plasmids/genetics Precipitin Tests Protein Serine-Threonine Kinases/genetics,metabolism Saccharomyces cerevisiae/genetics Transcription Factors/genetics,metabolism Two-Hybrid System Techniques
Chemicals
NM23 Nucleoside Diphosphate Kinases Transcription Factors AURKA protein, human Aurora Kinase A Aurora Kinases Protein Serine-Threonine Kinases NME1 protein, human Nucleoside-Diphosphate Kinase Monomeric GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Du Jian
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Hannon Gregory J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkf678
Published
2002-12-15
Pages
5465-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC140054
Subset
IM
Grants
NCI NIH HHS · P01 CA013106 · United States
NCI NIH HHS · P01-CA13106 · United States
External Links
PubMed source DOI: 10.1093/nar/gkf678 PMC Full Text View journal details
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