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PMID: 10559879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A.

Nature cell biology ·Vol. 1 ·No. 2 ·1999-06-00 ·Pages 88-93

Meraldi P, Lukas J, Fry AM, Bartek J, Nigg EA

Abstract

Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2-cyclin A activity.

MeSH Terms
Animals CDC2-CDC28 Kinases CHO Cells Carrier Proteins Cell Cycle/physiology Cell Cycle Proteins/metabolism Centrosome/physiology,ultrastructure Cricetinae Cyclin A/metabolism Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases/metabolism DNA Replication DNA-Binding Proteins E2F Transcription Factors Mammals Protein Serine-Threonine Kinases/metabolism Recombinant Proteins/metabolism Retinoblastoma-Binding Protein 1 Transcription Factors/metabolism Transfection
Chemicals
Carrier Proteins Cell Cycle Proteins Cyclin A DNA-Binding Proteins E2F Transcription Factors Recombinant Proteins Retinoblastoma-Binding Protein 1 Transcription Factors Protein Serine-Threonine Kinases CDC2-CDC28 Kinases Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Meraldi P
Department of Molecular Biology, University of Geneva, Switzerland.
Lukas J
Fry A M
Bartek J
Nigg E A
Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1465-7392
DOI
10.1038/10054
Published
1999-06-00
Pages
88-93
Language
English
Region
England
NLM ID
100890575
Subset
IM
External Links
PubMed source DOI: 10.1038/10054 View journal details
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